MyoD Antibody (5.8A) [PE]

• Reactivity: Hu, Mu, Rt, Po
• Applications: WB, Flow, ICC/IF, IHC, IP, KO, Mycoplasma
• Clone: 5.8A
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

MyoD Antibody (5.8A) [PE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
• Specificity: In Rh-30, a ~45 kDa band should be observed.
• Predicted Species: Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot
• Application Notes: There is considerable literature published using the MyoD, Clone 5.8 antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.
  
  Recommended applications are based on validated applications from the unconjugated base product NB100-56511. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MyoD Antibody (5.8A) [PE]

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYOD1