Myocilin Antibody (297823) [Allophycocyanin]

HEK293 cell line transfected with hMYOC vs irrelevant HEK293 transfectant cells were stained with Mouse Anti-Human Myocilin APC‑conjugated Monoclonal Antibody (Catalog # FAB34461A, filled histogram) or isotype control antibody, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry Fixation Buffer (Catalog # FC004). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 297823
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Myocilin Antibody (297823) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Myocilin
  Arg33-Met504
  Accession # Q99972
• Specificity: Detects human Myocilin in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myocilin Antibody (297823) [Allophycocyanin]

• GLC1A
• GLC1Amyocilin
• GPOA
• JOAG
• JOAG1
• MYOC
• Myocilin
• myocilin, trabecular meshwork inducible glucocorticoid response
• TIGR
• TIGRmutated trabecular meshwork-induced glucocorticoid response protein
• Trabecular meshwork-induced glucocorticoid response protein

Background

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q99972()