Fast skeletal myosin light chain 1 Antibody

Images

 
Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Sample (whole cell lysate) A: 1ug mouse heart, B: 5ug mouse heart, 15% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Sample (1ug of whole cell lysate) A: Rat muscle 15% SDS PAGE gel, diluted at 1:10000.
Western Blot: Fast skeletal myosin light chain 1 Antibody [NBP2-16433] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.59 mg/ml

Order Details

Fast skeletal myosin light chain 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Fast skeletal myosin light chain 1. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Chicken (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.59 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fast skeletal myosin light chain 1 Antibody

  • A1 catalytic
  • A2 catalytic
  • MLC1/MLC3
  • MLC1F
  • MLC1F/MLC3F
  • MLC3F
  • myosin light chain 1/3, skeletal muscle isoform
  • Myosin light chain A1/A2
  • Myosin light chain alkali 1/2
  • myosin, light chain 1, alkali; skeletal, fast
  • myosin, light polypeptide 1, alkali; skeletal, fast

Background

Myosin is a hexameric ATPase cellular motor protein. It is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene encodes a myosin alkali light chain expressed in fast skeletal muscle. Two transcript variants have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYL1
Uniprot