Myeloperoxidase/MPO Antibody (59A5)

Product Discontinued

Myeloperoxidase/MPO Antibody (59A5) Summary

• Immunogen: Recombinant protein from exon 7 of the human myeloperoxidase.
• Localization: Cytoplasmic
• Specificity: Myeloperoxidase metabolizes most of the hydrogen peroxide generated by activated phagocytes. This stains strongly with the cytoplasm of myeloid cells. The antibody detects myeloperoxidase found in myeloid cells of acute myeloid leukemia. The myeloperoxidase gene has been localized to chromosome 17.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Neat
• Application Notes: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded tissue sections. It is not suitable for frozen tissue.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: No buffer
• Preservative: Sodium Azide and Proclin 300
• Purity: Tissue culture supernatant

Alternate Names for Myeloperoxidase/MPO Antibody (59A5)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MPO