![Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with a](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Western-Blot-NBP2-72846-img0001.jpg "Western Blot: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Myeloid leukemia factor 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with a")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI3A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human MLF1(NP_071888) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MLF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 30.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for Myeloid leukemia factor 1 Antibody (NBP2-72846)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MLF1 |
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Human pancreas tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0009.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Human pancreas tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72846-img0003.jpg "Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0004.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0005.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0006.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0007.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0008.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Human tonsil using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72846-img0010.jpg "Immunohistochemistry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - Staining of paraffin-embedded Human tonsil using anti-Myeloid leukemia factor 1 mouse monoclonal antibody.")
![Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - HEK293T cells transfected with either Myeloid leukemia factor 1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MLF1 antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Myeloid-leukemia-factor-1-Antibody-OTI3A3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72846-img0002.jpg "Flow Cytometry: Myeloid leukemia factor 1 Antibody (OTI3A3) - Azide and BSA Free [NBP2-72846] - HEK293T cells transfected with either Myeloid leukemia factor 1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MLF1 antibody , and then analyzed by flow cytometry.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")