Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free

Immunohistochemistry-Paraffin: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534] - Formalin-fixed, paraffin-embedded human brain stained with Myelin PLP Mouse Monoclonal Antibody (PLP1/4259).

Flow Cytometry: Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free [NBP3-08534] - Total extracellular vesicles with Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] (Catalog # NBP3-08534AF647) (left). Gate determined vesicles positive for PLP1 antibody ranging from 500-800nm size. Image on the right represents the same sample without antibody. 10ul of pre-isolated extracellular vesicles were incubated with 2ul of Myelin PLP Antibody (PLP1/4259) [Alexa Fluor® 647] , during 20 minutes at room temperature, and analyzed in nanoscale flow cytometer able to resolve particles scattering as little as 100um. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt, Bv, Re
• Applications: Flow, IHC
• Clone: PLP1/4259
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07603).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Synthetic peptide GRGTKF that recognizes the C terminal region (aa272-277) of Myelin PLP protein. (Uniprot: P60201)
• Localization: Membrane
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLP1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Validated for Flow Cytometry from a verified customer review.
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Reactivity Notes

Tenerife Lizard

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Myelin PLP Antibody (PLP1/4259) - Azide and BSA Free

• DM-20
• HLD1
• Lipophilin
• major myelin proteolipid protein
• MMPL
• Myelin PLP
• myelin proteolipid protein
• PLP
• PLP/DM20
• PLP1
• PMD
• proteolipid protein 1
• spastic paraplegia 2, uncomplicated
• SPG2

Background

Human PLP (also known as lipophilin) is a 30 kDa protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in compaction. Different mutations of the human PLP gene product result in two neurological disorders - Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLP1