Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) - BSA Free

Flow Cytometry: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) [NBP3-27131] - Analysis of HL-60 cells using Muscarinic Acetylcholine Receptor M5/CHRM5 mouse mAb (green) and negative control (red).

Western Blot: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) [NBP3-27131] - Analysis using Muscarinic Acetylcholine Receptor M5/CHRM5 mAb against human Muscarinic Acetylcholine Receptor M5/CHRM5 recombinant protein. (Expected MW is 37.4 kDa)

ELISA: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) [NBP3-27131] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) [NBP3-27131] - Analysis using Muscarinic Acetylcholine Receptor M5/CHRM5 mAb against HEK293 (1) and Muscarinic Acetylcholine Receptor M5/CHRM5-hIgGFc transfected HEK293 (2) cell lysate.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 7E5H11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Muscarinic Acetylcholine Receptor M5/CHRM5 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRM5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 42.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody (7E5H11) - BSA Free

• cholinergic receptor, muscarinic 5
• CHRM5
• HM5
• M5
• M5R
• mAChR M5
• MGC41838
• muscarinic acetylcholine receptor M5

Background

Muscarinic cholinergic receptor M5 is an Acetylcholine Receptor that is active during embryonic development. It is coupled to phosphoinositide degradation and to the activation of mitogen-activated protein kinase (MAPK). The M5 receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRM5