5-HT3A Antibody


Biological Strategies: Western Blot: 5-HT3A Antibody [NB100-56351] - The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS ...read more
Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/mL.
Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A: human, B: mouse and C: rat brain tissue lysate using 5-HT3A antibody at 3 ug/mL.
Simple Western: 5-HT3A Antibody [NB100-56351] - Image shows a specific band for 5HT3A in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
The expression of 5-HT3A receptor increased in the E2 + FS group compared to that in the oil + non-FS group. *P < 0.05 versus the oil + non-FS group. E2: estradiol; FS: forced swim.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

5-HT3A Antibody Summary

This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE) of rat 5-HT3 Receptor, accession number P35563.
Protein G purified
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  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Simple Western 1:50
  • Western Blot 3 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human Brain Whole Tissue Lysate (Adult Whole Normal)
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NB100-56351 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for 5-HT3A Antibody

  • 5-HT-3,5-HT3-A
  • 5HT3A
  • 5-HT3A
  • 5-HT3R5-hydroxytryptamine receptor 3A
  • 5-hydroxytryptamine (serotonin) receptor 3A
  • 5-hydroxytryptamine receptor 3,5-HT3A5HT3R
  • HTR35HT3 serotonin receptor
  • HTR3A
  • Serotonin receptor 3A
  • Serotonin-gated ion channel receptor


Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for 5-HT3A Antibody (NB100-56351)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: IHC-FrFl, WB.

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Showing Publications 1 - 7 of 7.
Publications using NB100-56351 Applications Species
Mo SY, Xue Y, Li Y et al. Descending serotonergic modulation from rostral ventromedial medulla to spinal trigeminal nucleus is involved in experimental occlusal interference-induced chronic orofacial hyperalgesia The journal of headache and pain 2023-05-10 [PMID: 37165344] (WB, IHC-FrFl, Rat) WB, IHC-FrFl Rat
Tao ZY, Qiu XY, Wei SQ et al. SAHA Inhibits Somatic Hyperalgesia Induced by Stress Combined with Orofacial Inflammation Through Targeting Different Spinal 5-HT Receptor Subtypes Neurochemical research 2022-01-29 [PMID: 35092569]
Huang Y, Huang J, Zhou QX et al. ZFP804A mutant mice display sex-dependent schizophrenia-like behaviors Molecular psychiatry 2020-12-10 [PMID: 33303946] (WB, Mouse) WB Mouse
Li JH, Yang JL, Wei SQ et al. Contribution of central sensitization to stress-induced spreading hyperalgesia in rats with orofacial inflammation Mol Brain 2020-07-28 [PMID: 32723345] (WB, Rat) WB Rat
Li ZL, Xue Y, Tao ZY et al. Spinal 5-HT3 Receptor Contributes to Somatic Hyperalgesia Induced by Sub-chronic Stress Mol Pain. 2019-06-10 [PMID: 31184246] (WB, Rat) WB Rat
Stegemann A, Bohm M. Tropisetron via a7 nicotinic acetylcholine receptor suppresses tumor necrosis factor-a-mediated cell responses of human keratinocytes. Exp. Dermatol. 2019-01-17 [PMID: 30653770] (WB, Human) WB Human
Nguyen H, Wang H, le T et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil. 2008-03-01 [PMID: 17919312] (WB, Rat)

5-HT3 Receptor (IMG-367). WB: Rat hypothalmus. FIG 3. Sold through distributor.
WB Rat

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FAQs for 5-HT3A Antibody (NB100-56351). (Showing 1 - 1 of 1 FAQs).

  1. Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452
    • We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.

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Gene Symbol HTR3A