Western Blot: 5-HT3A Antibody [NB100-56351] - Analysis of 5-HT3A in A) human, B) mouse and C) rat brain tissue lysate using 5-HT3A antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: 5-HT3A Antibody [NB100-56351] - Analysisof 5-HT3A in human testis using 5-HT3A antibody at 5 ug/ml.
Simple Western: 5-HT3A Antibody [NB100-56351] - Simple Western lane view shows a specific band for 5HT3A in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE) of rat 5-HT3 Receptor, accession number P35563.
In human brain, a 47-50 kDa band is obeserved.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB100-56351 in the following applications:
Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor plays important roles in regulating diverse biological processes in central and peripheral nervous systems, cardiovascular systems, and gastrointestinal systems. The 5-HT3 receptors are distinguished from other 5-HT receptors by their structure and function. 5-HT3 receptors span the membrane only four times and are ligand-gated, cation-selective ion channels that mediate neuronal excitation and exocytotic release of neurotransmitters within the peripheral and central nervous systems. Functional studies done on isolated tissues have revealed heterogeneity of this receptor. The human 5-HT3R-As cDNA contains an open reading frame of 1434 base pairs predicting a 23-residue signal peptide and a mature receptor subunit of 455 amino acids. The human subunit exhibits 85% and 84% amino acid sequence identity to the mouse and rat receptors, respectively. Anti-5-HT3R polyclonal antibody shows immunoreactivity in the forebrain (olfactory regions, hippocampal formation and amygdala), sensory and motor nuclei of brainstem and spinal cord.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for 5-HT3A Antibody (NB100-56351). (Showing 1 - 1 of 1 FAQ).
Can you please let me know if you have antibodies against the following? For nAChR alpha 7 receptor of human origin - between amino acids 321- 465 For Glycine alpha1 receptor of human origin - between amino acids 339-413 For 5-HT 3A receptor of mouse origin - between amino acids 338-452
We do not routinely epitope map our antibodies, and do not typically know where the antigen/antibody cites are. On occasion, customers will provide feedback about the epitopes, but this is not the norm. Unfortunately, we do not have any antibodies for nAChR alpha 7 receptor. For alpha 1 Glycine Receptor, we have two antibodies that target the N-terminus, NB300-113 and NBP1-49174, but I do not have more specific information about the epitope. For 5HT3A, we do have one antibody that may suit your needs, NB100-56351.