Actin Alpha 1 Cardiac Muscle Antibody (22D3)

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo

• Reactivity: Hu, Mu, Rt, Po, Bv, Gt, Ma, Rb, Xp, Ze
• Applications: WB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 22D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Actin Alpha 1 Cardiac Muscle Antibody (22D3) Summary

• Immunogen: Clone 22D3 is a mouse monoclonal IgG1 antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-cardiac actin with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
• Specificity: Clone 22D3 is an antibody highly specific for a- cardiac actin, and does not cross react with other actin isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Application Notes: Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3)

• ACTC
• ACTC1
• actin, alpha, cardiac muscle 1
• alpha-Cardiac Actin
• ASD5
• Cardiac alpha-Actin
• CMD1R
• CMH11
• LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)(2)

Publications using NBP1-97725

• Domke LM, Franke WW. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system Cell Tissue Res. Dec 27 2018 [PMID: 30591979] (ICC/IF, Bovine)
• Chen Chen, Yang Shenglan, Li Huaping et al. Mir30c Is Involved in Diabetic Cardiomyopathy through Regulation of Cardiac Autophagy via BECN1. Molecular Therapy. Nucleic Acids 2017 Mar 29 [PMID: 28624189] (WB, Mouse)

Review for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Ran Guo IF Other 10/13/2015

Summary

• Application: Immunofluorescence
• Sample Tested: embryos at stage 46
• Species: Other
• Lot: 81358

Blocking

• Blocking Details: blocking with 5% BSA for one hour at RT.

Primary Anitbody

• Dilution Ratio: diluting antibody with 5% BSA at 1:200, incubating at RT for two hours.

Details

• Detection Notes: Sections were imaged using a Nikon 90I fluorescence microscope.
• Fixation Details: X. laevis embryos were fixed and stored in 4% paraformaldehyde (in PBS) at 4℃ overnight. After dehydration in 20% and 30% sucros

Bioinformatics

• Gene Symbol: ACTC1