Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle ...read more
Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Gt, Rb, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
22D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free Summary

Immunogen
Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity
This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:5000
Application Notes
This antibody is suitable for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97725 in the following applications:

Publications
Read Publications using
NBP1-97725 in the following applications:

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Notes

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

  • ACTC
  • ACTC1
  • actin, alpha, cardiac muscle 1
  • alpha-Cardiac Actin
  • ASD5
  • Cardiac alpha-Actin
  • CMD1R
  • CMH11
  • LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)(2)

We have publications tested in 2 confirmed species: Mouse, Bovine.

We have publications tested in 2 applications: ICC/IF, WB.


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Review for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-97725:
Filter by Applications
Immunofluorescence
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Actin Alpha 1 Cardiac Muscle NBP1-97725
Enlarge
5
reviewed by:
Verified Customer
Immunofluorescence Other 10/13/2015
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Summary

ApplicationImmunofluorescence
Sample Testedembryos at stage 46
SpeciesOther
Lot81358

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Video Protocols

WB Video Protocol
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Recent Reviews

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Verified Customer
10/13/2015
Application: Immunofluorescence
Species: Other

Bioinformatics

Gene Symbol ACTC1