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Actin Alpha 1 Cardiac Muscle Antibody (22D3)

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 (1 Review)
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Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle ...read more
Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo
Immunocytochemistry/ Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Gt, Ma, Rb, Xp, ZeSpecies Glossary
Applications WB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
22D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-97725AF350
Alexa Fluor 405
NBP1-97725AF405
Alexa Fluor 488
NBP1-97725AF488
Alexa Fluor 532
NBP1-97725AF532
Alexa Fluor 594
NBP1-97725AF594
Alexa Fluor 647
NBP1-97725AF647
Alexa Fluor 700
NBP1-97725AF700
Alexa Fluor 750
NBP1-97725AF750
DyLight 350
NBP1-97725UV
DyLight 405
NBP1-97725V
DyLight 488
NBP1-97725G
DyLight 550
NBP1-97725R
DyLight 594
NBP1-97725DL594
DyLight 650
NBP1-97725C
DyLight 680
NBP1-97725FR
DyLight 755
NBP1-97725IR
Janelia Fluor 549
NBP1-97725JF549
Janelia Fluor 646
NBP1-97725JF646
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Actin Alpha 1 Cardiac Muscle Antibody (22D3) Summary

Immunogen
Clone 22D3 is a mouse monoclonal IgG1 antibody derived by fusion of NS0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a peptide comprising the N-terminal nonapeptide of alpha-cardiac actin with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity
Clone 22D3 is an antibody highly specific for a- cardiac actin, and does not cross react with other actin isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ACTC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:1000-1:5000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97725 in the following applications:
Publications
Read Publications using
NBP1-97725 in the following applications:

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3)

  • ACTC
  • ACTC1
  • actin, alpha, cardiac muscle 1
  • alpha-Cardiac Actin
  • ASD5
  • Cardiac alpha-Actin
  • CMD1R
  • CMH11
  • LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: MYH7 Antibody [NBP2-94079] - Analysis of rat skeletal muscle using MYH7 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (2C9) [NBP2-35336] - Formalin fixed tissue section of human breast with 5 ug/mL concentration of Troponin I Type 3 (cardiac) antibody (clone 2C9) showing no staining - Negative Control
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody (2C9) [NBP2-35336] - Analysis of FFPE tissue section of human heart using Troponin I antibody (clone 2C9) at 5 ug/mL concentration. The antibody specifically detected the target protein in cardiac muscle fibers in the tested sections, whereas, the nuclei as well as the intercellular collagen network did not develop any immunostaining.
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Troponin T was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Troponin T was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human Troponin T (Cardiac) Monoclonal Antibody (Catalog # MAB1874) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunohistochemistry-Paraffin: MyBPC3 Antibody [NBP2-13631] - Staining of human prostate shows no positivity in smooth muscle cells or glandular cells as expected.
Immunohistochemistry-Paraffin: MyBPC3 Antibody [NBP2-13631] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: MyBPC3 Antibody [NBP2-13631] - Staining of human prostate shows low expression as expected.
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Immunohistochemistry-Paraffin: Myosin light chain 3 Antibody [NBP1-88068] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in cardiomyocytes.
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Myosin Light Chain
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Western Blot: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MYL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Mouse heart tissue lysate
Immunocytochemistry/Immunofluorescence: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: Myosin Light Chain 2 Antibody (3B2) [NBP1-30249] - Analysis of MYL2 in A431 cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
(1 Review)
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Immunohistochemistry-Paraffin: Troponin C (cardiac) Antibody [NBP2-37958] - Staining of human heart muscle shows high expression.
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Western Blot: Tafazzin/TAZ Antibody (1B10) [H00006901-M12] - Analysis of TAZ expression in SW-13 (Cat # L005V1).
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Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)(2)

We have publications tested in 2 confirmed species: Mouse, Bovine.

We have publications tested in 2 applications: ICC/IF, WB.


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Review for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-97725:
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence Actin Alpha 1 Cardiac Muscle NBP1-97725
Enlarge 		5
reviewed by:
Ran Guo
 IF Other 10/13/2015
View

Summary

ApplicationImmunofluorescence
Sample Testedembryos at stage 46
SpeciesOther
Lot81358

Blocking

Blocking Detailsblocking with 5% BSA for one hour at RT.

Primary Anitbody

Dilution Ratiodiluting antibody with 5% BSA at 1:200, incubating at RT for two hours.

Details

Detection NotesSections were imaged using a Nikon 90I fluorescence microscope.
Fixation DetailsX. laevis embryos were fixed and stored in 4% paraformaldehyde (in PBS) at 4℃ overnight. After dehydration in 20% and 30% sucros

Product General Protocols

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WB Video Protocol
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FAQs for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-97725AF350
Alexa Fluor 405 NBP1-97725AF405
Alexa Fluor 488 NBP1-97725AF488
Alexa Fluor 532 NBP1-97725AF532
Alexa Fluor 594 NBP1-97725AF594
Alexa Fluor 647 NBP1-97725AF647
Alexa Fluor 700 NBP1-97725AF700
Alexa Fluor 750 NBP1-97725AF750
DyLight 350 NBP1-97725UV
DyLight 405 NBP1-97725V
DyLight 488 NBP1-97725G
DyLight 550 NBP1-97725R
DyLight 594 NBP1-97725DL594
DyLight 650 NBP1-97725C
DyLight 680 NBP1-97725FR
DyLight 755 NBP1-97725IR
Janelia Fluor 549 NBP1-97725JF549
Janelia Fluor 646 NBP1-97725JF646
Learn about Custom Antibody Labeling

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Diseases for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)

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Recent Reviews

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Immunofluorescence Actin Alpha 1 Cardiac Muscle NBP1-97725
Enlarge
 Ran Guo
10/13/2015
Application: IF
Species: Other
read this review

Bioinformatics

Gene Symbol ACTC1