Reactivity | Hu, Mu, Rt, Po, Bv, Gt, Rb, ZeSpecies Glossary |
Applications | WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P |
Clone | 22D3 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Format | BSA Free |
Concentration | 1 mg/ml |
Immunogen | Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue. |
Specificity | This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | ACTC1 |
Purity | Protein A or G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This antibody is suitable for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS, pH 7.2 |
Preservative | 0.09% Sodium Azide |
Concentration | 1 mg/ml |
Purity | Protein A or G purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
Immunofluorescence | Other | 10/13/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)Discover more about diseases related to Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725).
| Pathways for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)View related products by pathway.
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Verified Customer 10/13/2015 |
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Application: | Immunofluorescence | |
Species: | Other |
Gene Symbol | ACTC1 |