Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing somites in stage 46 Xenopus laevis embryo using Actin Alpha 1 Cardiac Muscle Antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Paraffin section of human cardiac muscle immunostained with 22D3 (1:500)

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of developing myocardium in 1 month old zebrafish embryo

• Reactivity: Hu, Mu, Rt, Po, Bv, Gt, Rb, Ze
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 22D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free Summary

• Immunogen: Derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of Actin Alpha 1 Cardiac Muscle with an acetylated N-terminus (Ac-DDEETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
• Specificity: This antibody is highly specific for actin alpha 1 (cardiac), and does not cross react with other actin isoforms.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTC1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:5000
• Application Notes: This antibody is suitable for immunohistochemistry on frozen and paraffin-embedded tissues, immunoblotting and ELISA. Optimal antibody dilutions should be determined by titration; recommended range is 1:100 - 1:1000 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:1000 - 1:5000 for immunoblotting applications.

Reactivity Notes

The epitope recognized by this antibody is highly conserved; the antibody therefore cross-reacts with many species

Bovine reactivity reported in scientific literature (PMID: 30591979). Mouse reactivity reported in scientific literature (PMID: 28624189).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.2
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Notes

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Alternate Names for Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

• ACTC
• ACTC1
• actin, alpha, cardiac muscle 1
• alpha-Cardiac Actin
• ASD5
• Cardiac alpha-Actin
• CMD1R
• CMH11
• LVNC4

Background

Actin, alpha cardiac muscle 1 (ACTC1) is a 377 aa cytoskeletal filament in cardiac muscle which interacts with the 50-KDa domain of the myosin motor domain in cardiac muscle contraction. Actin is highly conserved across species, and differs from skeletal muscle actin (ACTA1) by only four amino acids. Mutations in the ACTC1 gene are linked to familial hypertrophy cardiomyopathy, atrial septal defects, arrhythmia, and chronic inflammatory cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725)(2)

Publications using NBP1-97725

• Domke LM, Franke WW. The cell-cell junctions of mammalian testes: II. The lamellar smooth muscle monolayer cells of the peritubular wall are laterally connected by vertical adherens junctions-a novel architectonic cell-cell junction system Cell Tissue Res. Dec 27 2018 [PMID: 30591979] (ICC/IF, Bovine)
• Chen Chen, Yang Shenglan, Li Huaping et al. Mir30c Is Involved in Diabetic Cardiomyopathy through Regulation of Cardiac Autophagy via BECN1. Molecular Therapy. Nucleic Acids 2017 Mar 29 [PMID: 28624189] (WB, Mouse)

Review for Actin Alpha 1 Cardiac Muscle Antibody (NBP1-97725) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer Immunofluorescence Other 10/13/2015

Summary

• Application: Immunofluorescence
• Sample Tested: embryos at stage 46
• Species: Other
• Lot: 81358

Bioinformatics

• Gene Symbol: ACTC1