Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human lymph node, Hodgkins lymphoma.
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human brain, neurons and glia.
Product Details
Summary
Reactivity
Hu, Mu, Rt, Po, Bv, Eq, Gp, Ha, Bt, Ca, PmSpecies Glossary
Predicted cross-reactivity based on sequence identity: Gorilla (100%).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody
cholinergic receptor, muscarinic 4
CHRM4
HM4
M4
M4R
mAChR M4
muscarinic acetylcholine receptor M4
Background
Muscarinic acetylcholine receptor M4 is an Acetylcholine Receptor. Unlike muscarinic receptor M2, the M4 receptor is not involved in smooth muscle contraction. The clinical relevance of this receptor is unknown. However, mouse studies have linked it to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus. ESTs have been isolated from human breast and eye libraries.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human lymph node, Hodgkins lymphoma.](http://images.novusbio.com/fullsize2/Muscarinic-Acetylcholine-Receptor-M4-CHRM4-Antibody-Immunohistochemistry-Paraffin-NLS220-img0002.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human lymph node, Hodgkins lymphoma.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize2/Muscarinic-Acetylcholine-Receptor-M4-CHRM4-Antibody-Immunohistochemistry-Paraffin-NLS220-img0001.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human brain, neurons and glia.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining in human cerebral cortex and pancreas tissues using anti-CHRM1 antibody. Corresponding CHRM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of dissociated rat hippocampal culture. Image submitted by a verified customer review.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-NBP1-87466-img0003.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP1-87466] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M1-CHRM1-Antibody-Immunohistochemistry-Paraffin-NBP1-87466-img0007.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Brain, Hypothalamus.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0006.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, neurons and glia.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1331] - Analysis of anti-CHRM2 / M2 antibody with human brain, glioblastoma.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1331-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NLS5259] - Brain, Alzheimer's disease neurofibrillary tangle](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M3-CHRM3-Antibody-Immunohistochemistry-Paraffin-NLS5259-img0009.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NLS5259] - Analysis of anti-CHRM3 / M3 antibody with human brain, glioblastoma.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M3-CHRM3-Antibody-Immunohistochemistry-Paraffin-NLS5259-img0004.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NLS5259] - Human heart formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M3-CHRM3-Antibody-Immunohistochemistry-Paraffin-NLS5259-img0005.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP2-94150] - Analysis of extracts of various cell lines, using Muscarinic Acetylcholine Receptor M5/CHRM5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/images2/Muscarinic Acetylcholine Receptor M5-CHRM5 Antibody-Western Blot-NBP2-94150-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP2-94150] - Analysis of U2OS cells using Muscarinic Acetylcholine Receptor M5/CHRM5. Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M5-CHRM5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94150-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP2-94150] - Paraffin-embedded rat brain using Muscarinic Acetylcholine Receptor M5/CHRM5.](http://images.novusbio.com/images2/Muscarinic-Acetylcholine-Receptor-M5-CHRM5-Antibody-Immunohistochemistry-Paraffin-NBP2-94150-img0002.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against HEK293 (1) and CHRNA4 (AA: 29-242)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0003.jpg)
![Flow Cytometry: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mouse mAb against SH-SY5Y (1) cell lysate.](http://images.novusbio.com/images2/Nicotinic Acetylcholine R alpha 4-CHRNA4 Antibody (7B4H1)-Flow Cytometry-NBP2-61674-img0004.jpg)
![Western Blot: Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibody (7B4H1) [NBP2-61674] - Analysis using CHRNA4 mAb against human CHRNA4 (AA: 29-242) recombinant protein. (Expected MW is 52.5 kDa)](http://images.novusbio.com/images2/Nicotinic-Acetylcholine-R-alpha-4-CHRNA4-Antibody-(7B4H1)-Western-Blot-NBP2-61674-img0002.jpg)
![Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human hypothalamus shows strong positivity in neuronal projections.](http://images.novusbio.com/images2/Cholecystokinin-Antibody-Immunohistochemistry-Paraffin-NBP2-62664-img0003.jpg)
![Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Analysis in human duodenum and skeletal muscle tissues using Anti-CCK antibody. Corresponding CCK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cholecystokinin-Antibody-Immunohistochemistry-Paraffin-NBP2-62664-img0001.jpg)
![Immunohistochemistry-Paraffin: Cholecystokinin Antibody [NBP2-62664] - Staining of human duodenum shows high expression.](http://images.novusbio.com/images2/Cholecystokinin-Antibody-Immunohistochemistry-Paraffin-NBP2-62664-img0002.jpg)
![Western Blot: Influenza A H5N1 Antibody (2B7) [NBP2-22605] - Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Influenza A H5N1/HA1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: H7N9-HA1(19-339aa) (A/Anhui/1-BALF_RG45/2013)Lane 2.: H7N9-HA1(1-523aa)(A/Shanghai/JS01/2013)Lane 3.: H5N1-HA1(17-338aa) (A/Vietnam/HN31242/2007)](http://images.novusbio.com/images2/Influenza-A-H5N1-Antibody-(2B7)-NBP2-22605-img0003.jpg)
![Western Blot: Influenza A H5N1 Antibody (2B7) [NBP2-22605] - H5N1/HA1 recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human H5N1/HA1 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/images2/Influenza A Virus Hemagglutinin Antibody (2B7)-Western Blot-NBP2-22605-img0002.jpg)
![ELISA: Influenza A H5N1 Antibody (2B7) [NBP2-22605] - Analysis using ELISA.](http://images.novusbio.com/images2/Influenza A Virus Hemagglutinin Antibody (2B7)-ELISA-NBP2-22605-img0001.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - Section of human skin tumor (higher magnification). Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:500. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.](http://images.novusbio.com/images2/BCL11B-Antibody-Immunohistochemistry-NB100-2600-img0005.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - P7 brain mouse section was stained with anti-BCL11B antibody. Image from verified customer review.](http://images.novusbio.com/images2/BCL11B-Antibody-Immunohistochemistry-Paraffin-NB100-2600-img0003.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NB100-2600] - Section of human skin tumor. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:500. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.](http://images.novusbio.com/images2/BCL11B-Antibody-Immunohistochemistry-NB100-2600-img0004.jpg)
![Western Blot: P2X4 Antibody [NBP2-27567] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/images2/P2X4-Antibody-Western-Blot-NBP2-27567-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: P2X4 Antibody [NBP2-27567] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/images2/P2X4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27567-img0003.jpg)
![Immunohistochemistry-Paraffin: P2X4 Antibody [NBP2-27567] - Staining of paraffin embedded Human Uterus. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/P2X4-Antibody-Immunohistochemistry-Paraffin-NBP2-27567-img0005.jpg)
![Western Blot: U11/U12-35K Antibody [NBP2-20774] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/U11-U12-35K Antibody-Western Blot-NBP2-20774-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: U11/U12-35K Antibody [NBP2-20774] - U11/U12-35K antibody detects U11/U12-35K protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: U11/U12-35K protein stained by U11/U12-35K antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/U11-U12-35K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20774-img0004.jpg)
![Immunohistochemistry-Paraffin: U11/U12-35K Antibody [NBP2-20774] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/U11-U12-35K Antibody-Immunohistochemistry-Paraffin-NBP2-20774-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/images2/nNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB100-858-img0002.jpg)
![Immunohistochemistry-Frozen: nNOS Antibody [NB100-858] - Immunostaining of NOS1 neurons in cryosection of a perfusion-fixed (4% PFA) mouse hypothalamus. HRP-staining with Ni-DAB, after Biotin-SP-anti-goat (IgG) method. Data obtained by Drs. Eva Rumpler and Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary.](http://images.novusbio.com/images2/nNOS-Antibody-Immunohistochemistry-Frozen-NB100-858-img0004.jpg)
![Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/nNOS-Antibody-Flow-Cytometry-NB100-858-img0003.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/images2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0010.jpg)
![Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Heart tissue.](http://images.novusbio.com/images2/iNOS-Antibody-Immunohistochemistry-Paraffin-NB300-605-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/GluR2-Antibody-(JU20-43)-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0002.jpg)
![Immunohistochemistry-Paraffin: GluR2 Antibody (JU20-43) [NBP2-75510] - Analysis of paraffin-embedded mouse brain tissue using anti-GluR2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/GluR2-Antibody-(JU20-43)-Immunohistochemistry-Paraffin-NBP2-75510-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GluR2 Antibody (JU20-43) [NBP2-75510] - Staining GluR2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/GluR2-Antibody-(JU20-43)-Immunocytochemistry-Immunofluorescence-NBP2-75510-img0001.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")