![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human lymph node, Hodgkins lymphoma.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M4-CHRM4-Antibody-Immunohistochemistry-Paraffin-NLS220-img0002.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human lymph node, Hodgkins lymphoma.")
| Reactivity | Hu, Rt, Po, Bv, Eq, Gp, Ha, Mu, Ca, PmSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Product can be stored undiluted at 4C for up to 1 month. |
| Immunogen | Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human Muscarinic Acetylcholine Receptor M4/CHRM4. |
| Epitope | 3rd cytoplasmic domain |
| Specificity | Human CHRM4. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Predicted Species | Mouse (100%), Canine (95%), Primate (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CHRM4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in ICC/IF reported in scientific literature (PMID: 23212463).. |
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| Storage | Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]](https://images.novusbio.com/images2/CHRM3_MAB6378_Flow_Cytometry_24142.jpg)
![Immunohistochemistry Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (580011) [Unconjugated]](https://images.novusbio.com/images2/mab6378_human-chrm3-mab-clone-580011-immunohistochemistry-352020175913.jpg)
| Publication using NLS220 | Applications | Species |
|---|---|---|
| Fong G, Backman LJ, Andersson G et al. Human tenocytes are stimulated to proliferate by acetylcholine through an EGFR signalling pathway Cell Tissue Res 2012-12-05 [PMID: 23212463] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M4-CHRM4-Antibody-Immunohistochemistry-Paraffin-NLS220-img0001.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M4/CHRM4 Antibody [NLS220] - Analysis of anti-CHRM4 / M4 antibody with human brain, neurons and glia.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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