Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1)

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against human CHRNA7 (AA: extra 52-259) recombinant protein. (Expected MW is 50.4 kDa)

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of rectum cancer tissues using CHRNA7 mouse mAb with DAB staining.

Flow Cytometry: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of SH-SY5Y cells using CHRNA7 mouse mAb (green) and negative control (red).

Western Blot: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis using CHRNA7 mAb against HEK293 (1) and CHRNA7 (AA: extra 52-259)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Analysis of bladder cancer tissues using CHRNA7 mouse mAb with DAB staining.

ELISA: Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) [NBP2-61739] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-P
• Clone: 7F10G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1) Summary

• Immunogen: Purified recombinant fragment of human CHRNA7 (AA: extra 52-259) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHRNA7
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
• Theoretical MW: 56.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (7F10G1)

• a7 nicotinic acetylcholine receptor
• alpha 7 neuronal nicotinic acetylcholine receptor
• alpha-7 nicotinic cholinergic receptor subunit
• cholinergic receptor, nicotinic, alpha 7
• cholinergic receptor, nicotinic, alpha polypeptide 7
• CHRNA7
• CHRNA7-2
• NACHRA7
• neuronal acetylcholine receptor protein, alpha-7 chain
• neuronal acetylcholine receptor subunit alpha-7
• Nicotinic Acetylcholine R alpha 7
• Nicotinic Acetylcholine Ra 7

Background

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Nicotinic Acetylcholine R alpha 7/CHRNA7 Antibody (NBP2-61739). (Showing 1 - 2 of 2 FAQ).

1. I am doing research on Neuroscience and I would like to use some antibodies, especially in Western Blots, but I could do other assays: - alpha7 nicotinic cholinergic receptor; - parvalbumin; - calbindin D28K; The preferred reactivity is anti-human and anti-mouse. Do you have these antibodies? Are they in stock? And what about the prizes: How much are they? How much are the shipping costs? Could you make me any offer?
   • We currently have three alpha7 nicotinic cholingergic receptor antibodies available for purchase. Please see this list for our Parvalbumin antibodies. None are currently validated in mouse. I would like to introduce you to our Innovators Reward Program. We have an excelent calbinin D28K antibody that has been validated for use in human and mouse and WB. The pricing and availability of our products depends on your country.
2. I am looking to buy antibodies against most of the nicotinic receptor subunits for WB and IHC application. Could you please indicate which of your antibodies have been used by other researchers and provide publications showing their use? Thanks.
   • A list of our antibodies directed against he nicotinic acetylcholine receptors can be found here. Any products with publications will be indicated on the search page and can be found under the publications tab on the individual product's datasheet. Furthermore, we guarantee all of our products for the applications and species list on the datasheet. This should help narrow down your search.

Bioinformatics

• Gene Symbol: CHRNA7