Musashi-1 Antibody (3F2) - Azide and BSA Free

Western Blot: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of MSI1 expression in IMR-32 (Cat # L008V1).

Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of monoclonal antibody to MSI1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: Musashi-1 Antibody (3F2) [H00004440-M04] - Analysis of monoclonal antibody to MSI1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

ELISA: Musashi-1 Antibody (3F2) [H00004440-M04] - Detection limit for recombinant GST tagged MSI1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Musashi-1 Antibody (3F2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MSI1 (NP_002433, 1 a.a. ~ 87 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. METDAPQPGL ASPDSPHDPC KMFIGGLSWQ TTQEGLREYF GQFGE VKECL VMRDPLTKRS RGFGFVTFMD QAGVDKVLAQ SRHELDS
• Marker: Neuronal Stem Cell Marker
• Specificity: MSI1 (3F2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSI1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. FACS usage reported in scientific literature (PMID: 23818760).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Musashi-1 Antibody (3F2) - Azide and BSA Free

• MSI1
• Musashi (Drosophila) homolog 1
• musashi homolog 1 (Drosophila)
• Musashi1
• Musashi-1
• RNA-binding protein Musashi homolog 1

Background

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Musashi-1 Antibody (H00004440-M04)(3)

Publications using H00004440-M04

• Takashi H, Kenta F, Atsuko I et al. Thyroid hormone-induced expression of Foxl1 in subepithelial fibroblasts correlates with adult stem cell development during Xenopus intestinal remodeling. Sci Rep. 2020-11-26 [PMID: 33244068]
• Liu Xian, Yang Wen-Ting, Zheng Peng-Sheng. Msi1 promotes tumor growth and cell proliferation by targeting cell cycle checkpoint proteins p21, p27 and p53 in cervical carcinomas. Oncotarget. 2014-11-15 [PMID: 25362645] (ICC/IF)
• Lee HC, Ling QD, Yu WC et al. Drug-resistant colon cancer cells produce high carcinoembryonic antigen and might not be cancer-initiating cells. Drug Des Devel Ther. 2013-01-01 [PMID: 23818760] (FLOW)

Bioinformatics

• Gene Symbol: MSI1
• Entrez:
  • 4440(Human)
• OMIM:
  • 603328(Human)
• Uniprot:
  • NP_002433(Human)