MuRF1/TRIM63 Antibody (M316)

Western Blot: MuRF1/TRIM63 Antibody (M316) [NBP3-23424] - Western blot analysis of human full length MuRF1/TRIM63 recombinant protein. The blot was probed with mouse monoclonal MuRF1/TRIM63 (C-terminal region) at 1:250 (lane 1) and 1:1000 (lane 2) and rabbit polyclonal MuRF1/TRIM63 (C-terminal region) at 1:1000 (lanes 3) and 1:4000 (lane 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: M316
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MuRF1/TRIM63 Antibody (M316) Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Clone M316 was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal half of human MuRF1/TRIM63. This peptide sequence is highly conserved in rat and mouse MuRF1/TRIM63, and has 50% homology to MuRF2 (TRIM-55). [UniProt# Q969Q1]
• Specificity: The antibody detects a full length human MuRF1/TRIM63 recombinant protein on SDS-PAGE immunoblots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM63
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for MuRF1/TRIM63 Antibody (M316)

• EC 6.3.2.-
• IRF
• IRFMURF2
• Iris RING finger protein
• MuRF1
• MuRF-1
• muscle specific ring finger protein 2
• Muscle-specific RING finger protein 1
• RING finger protein 28FLJ32380
• RNF28
• RNF28E3 ubiquitin-protein ligase TRIM63
• SMRZ
• SMRZMURF-1
• Striated muscle RING zinc finger protein
• TRIM63
• tripartite motif containing 63
• tripartite motif-containing 63
• Tripartite motif-containing protein 63

Background

MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM63