Western Blot: Mucolipin 1 Antibody [NB110-82375] - Detection of Mucolipin-1 in mouse brain lysates using NB110-82375.
Simple Western: Mucolipin 1 Antibody [NB110-82375] - Simple Western lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more
This Mucolipin 1 antibody is useful for Western blot, where a band can be seen at approx. 66 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse and rat. Immunogen sequence has 85% homology to monkey and 78% homology to bovine.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for Mucolipin 1 Antibody
mucolipidosis type IV protein
Mucolipin 1 (MCOLN1, mucolipidin or TRPML1), is a member of TRP ion channels family and unlike most TRPs, mucolipin 1 primarily resides in the membranes of endocytic pathway (late endosomes/lysosomes). Widely expressed as multipass membrane protein, mucolipin 1's high expression levels are found in brain, liver and kidney. Mucolipin 1 activation by phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)], which is predominantly found in endocytic pathway and mucolipin 1 regulates the fusion/fission of endocytic pathway vesicles, and/or some aspect of lysosomal ion homeostasis such as pH, iron, or zinc content. It is a cation channel, and besides controlling membrane trafficking of proteins and lipids, it plays a major role in Ca2+ transport regulating lysosomal exocytosis. MCOLN1 mutation mediated mucolipin 1 down-regulation results in rare lysosomal storage disease mucolipidosis type IV (MLIV) that is characterized by progressive neurodegeneration with early onset, mental retardation, corneal opacity, retinal degeneration, vacuolization of parietal cells, constitutive achlorhydria and an elevated gastrin levels.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Mucolipin 1 Antibody (NB110-82375). (Showing 1 - 1 of 1 FAQ).
Has the Mucolipin 1 Antibody (NB110-82375) been used in any publications?
At this time NB110-82375 has not been cited in any publications, however a customer has provided a positive review for this product which you can view on the Images, Reviews & Publications tab of the product datasheet.
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