Mucolipin 1 Antibody

Images

 
Western Blot: Mucolipin 1 Antibody [NB110-82375] - Detection of Mucolipin-1 in mouse brain lysates using NB110-82375.
Simple Western: Mucolipin 1 Antibody [NB110-82375] - Simple Western lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.85 mg/ml

Mucolipin 1 Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of mouse Mucolipin 1 (within residues 500-580). [Swiss-Prot# Q99J21]
Localization
Cell membrane; Multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCOLN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1-0.5 ug/ml
  • Simple Western 1:40
Application Notes
This Mucolipin 1 antibody is useful for Western blot, where a band can be seen at approx. 66 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59657)
Control Peptide
Mucolipin 1 Peptide (NB110-82375PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB110-82375 in the following applications:

Reactivity Notes

Human, mouse and rat. Immunogen sequence has 85% homology to monkey and 78% homology to bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.85 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Mucolipin 1 Antibody

  • MG-2
  • ML4TRP-ML1
  • MLIV
  • MST080
  • MSTP080
  • mucolipidin
  • mucolipidosis type IV protein
  • mucolipin 1
  • mucolipin-1
  • TRPML1
  • TRP-ML1
  • TRPM-L1

Background

Mucolipin 1 (MCOLN1, mucolipidin or TRPML1), is a member of TRP ion channels family and unlike most TRPs, mucolipin 1 primarily resides in the membranes of endocytic pathway (late endosomes/lysosomes). Widely expressed as multipass membrane protein, mucolipin 1's high expression levels are found in brain, liver and kidney. Mucolipin 1 activation by phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P(2)], which is predominantly found in endocytic pathway and mucolipin 1 regulates the fusion/fission of endocytic pathway vesicles, and/or some aspect of lysosomal ion homeostasis such as pH, iron, or zinc content. It is a cation channel, and besides controlling membrane trafficking of proteins and lipids, it plays a major role in Ca2+ transport regulating lysosomal exocytosis. MCOLN1 mutation mediated mucolipin 1 down-regulation results in rare lysosomal storage disease mucolipidosis type IV (MLIV) that is characterized by progressive neurodegeneration with early onset, mental retardation, corneal opacity, retinal degeneration, vacuolization of parietal cells, constitutive achlorhydria and an elevated gastrin levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western

Publications for Mucolipin 1 Antibody (NB110-82375) (0)

There are no publications for Mucolipin 1 Antibody (NB110-82375).
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Review for Mucolipin 1 Antibody (NB110-82375) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-82375:
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WB
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Images Ratings Applications Species Date Details
 
reviewed by:
Math Cuajungco
WB Mouse 06/08/2011
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Summary

ApplicationWestern Blot
Sample TestedHEK lysate overexpressing mcoln1
SpeciesMouse
LotA1
CommentsThe antibody detects overexpressed mcoln1 gene.

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Details

Detection NotesSpecific Bands: 65 kDa

Comments

CommentsThe antibody detects overexpressed mcoln1 gene.

Product General Protocols

View specific protocols for Mucolipin 1 Antibody (NB110-82375): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Mucolipin 1 Antibody (NB110-82375). (Showing 1 - 1 of 1 FAQ).

  1. Has the Mucolipin 1 Antibody (NB110-82375) been used in any publications?
    • At this time NB110-82375 has not been cited in any publications, however a customer has provided a positive review for this product which you can view on the Images, Reviews & Publications tab of the product datasheet.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-82375AF405
Alexa Fluor 488 Labeled NB110-82375AF488
Alexa Fluor 647 Labeled NB110-82375AF647
Alexa Fluor 700 Labeled NB110-82375AF700
Biotin Labeled NB110-82375B
DyLight 405 Labeled NB110-82375V
DyLight 488 Labeled NB110-82375G
DyLight 550 Labeled NB110-82375R
DyLight 650 Labeled NB110-82375C
HRP Labeled NB110-82375H

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Bioinformatics Tool for Mucolipin 1 Antibody (NB110-82375)

Discover related pathways, diseases and genes to Mucolipin 1 Antibody (NB110-82375). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Mucolipin 1.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

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Recent Reviews

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Math Cuajungco
06/08/2011
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MCOLN1
Entrez
Uniprot