MUC5AC Antibody (SPM297) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fe, Mk, Rb, Bv(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SPM297
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

MUC5AC Antibody (SPM297) [Alexa Fluor® 700] Summary

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Localization
Cytoplasmic
Specificity
This MAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC5AC
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Reactivity Notes

Expected cross reactivity based on sequence identity: Hedgehog

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MUC5AC Antibody (SPM297) [Alexa Fluor® 700]

  • gastric mucin
  • leB
  • lewis B blood group antigen
  • major airway glycoprotein
  • MUC5
  • mucin 5, subtypes A and C, tracheobronchial/gastric
  • mucin 5AC, oligomeric mucus/gel-forming pseudogene
  • mucin 5AC, oligomeric mucus/gel-forming
  • mucin-5 subtype AC, tracheobronchial
  • mucin-5AC
  • TBM
  • tracheobronchial mucin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Mucin 5AC Antibody (NBP2-34758AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34758AF350
Alexa Fluor 405 NBP2-34758AF405
Alexa Fluor 488 NBP2-34758AF488
Alexa Fluor 532 NBP2-34758AF532
Alexa Fluor 594 NBP2-34758AF594
Alexa Fluor 647 NBP2-34758AF647
Alexa Fluor 700 NBP2-34758AF700
Alexa Fluor 750 NBP2-34758AF750
Allophycocyanin NBP2-34758APC
Biotin NBP2-34758B
DyLight 350 NBP2-34758UV
DyLight 405 NBP2-34758V
DyLight 488 NBP2-34758G
DyLight 550 NBP2-34758R
DyLight 594 NBP2-34758DL594
DyLight 650 NBP2-34758C
DyLight 680 NBP2-34758FR
DyLight 755 NBP2-34758IR
FITC NBP2-34758F
HRP NBP2-34758H
Janelia Fluor 549 NBP2-34758JF549
Janelia Fluor 646 NBP2-34758JF646
PE NBP2-34758PE
PerCP NBP2-34758PCP

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Calculators

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Bioinformatics

Gene Symbol MUC5AC