MTHFS Antibody

Western Blot: MTHFS Antibody [NBP1-20947] - (0.5ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947] - Staining of paraffin embedded Human Lung at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTHFS Antibody Summary

• Immunogen: Peptide with sequence KRCLQHQEVKPYT corresponding to internal region according to NP_006432.1.
• Epitope: internal region
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTHFS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in human liver lysates (calculated MW of 23.3 kDa band according to NP_006432.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTHFS Antibody

• 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase)
• 5-formyltetrahydrofolate cyclo-ligase
• EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase
• FLJ30410
• HsT19268
• Methenyl-THF synthetase

Background

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTHFS Antibody (NBP1-20947)(1)

Publication using NBP1-20947

• Anguera MC, Stover PJ. Methenyltetrahydrofolate synthetase is a high-affinity catecholamine-binding protein. Arch Biochem Biophys 2006-11-15 [PMID: 17055997]

Bioinformatics

• Gene Symbol: MTHFS
• Entrez:
  • 10588(Human)
  • 107885(Mouse)
• Uniprot:
  • NP_006432.1(Human)