TMED3 Antibody - Azide and BSA Free

Western Blot: TMED3 Antibody [NBP2-94321] - Analysis of extracts of various cell lines, using TMED3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 180s.

Immunohistochemistry-Paraffin: TMED3 Antibody [NBP2-94321] - Rat kidney using TMED3 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: TMED3 Antibody [NBP2-94321] - Human colon carcinoma using TMED3 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry-Paraffin: TMED3 Antibody [NBP2-94321] - Mouse lung using TMED3 antibody at dilution of 1:100 (40x lens).

Immunocytochemistry/ Immunofluorescence: TMED3 Antibody - Azide and BSA Free [TMED3] - Immunofluorescence analysis of A431 cells using TMED3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TMED3 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TMED3 Antibody - Azide and BSA Free (NBP2-94321) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-180 of human TMED3 (NP_031390.1). PCGAELTFELPDNAKQCFHEEVEQGVKFSLDYQVITGGHYDVDCYVEDPQGNTIYRETKKQYDSFTYRAEVKGVYQFCFSNEFSTFSHKTVYFDFQVGDEPPILPDMGNRVTALTQMESACVTIHEALKTVIDSQTHYRLREAQDRARAEDLNSRVS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMED3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for TMED3 Antibody - Azide and BSA Free

• C15orf22
• chromosome 15 open reading frame 22
• integral type I protein
• Membrane protein p24B
• MGC133022
• P24B
• transmembrane emp24 domain containing 3
• transmembrane emp24 domain-containing protein 3
• transmembrane emp24 protein transport domain containing 3,1200002G13Rik

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: TMED3