MTHFD2 Antibody

Images

 
Western Blot: MTHFD2 Antibody [NBP1-33200] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela E: Hep G2 F: Molt-4 G: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MTHFD2 Antibody [NBP1-33200] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded human breast carcinoma diluted at 1:500.
Immunohistochemistry-Paraffin: MTHFD2 Antibody [NBP1-33200] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: MTHFD2 Antibody [NBP1-33200] - Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 ug of MTHFD2 antibody [N1C3] Western blot analysis was performed using MTHFD2 antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.41 mg/ml

Order Details

MTHFD2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.
Localization
Mitochondrion
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTHFD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-33200 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (86%), Xenopus laevis (90%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.41 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTHFD2 Antibody

  • bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
  • methenyltetrahydrofolate cyclohydrolase
  • methylene tetrahydrofolate dehydrogenase (NAD+ dependent)
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2
  • mitochondrial
  • NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase
  • NMDMC

Background

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTHFD2 Antibody (NBP1-33200)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
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Filter By Species
Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MTHFD2 Antibody (NBP1-33200)

Discover related pathways, diseases and genes to MTHFD2 Antibody (NBP1-33200). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on MTHFD2.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

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Bioinformatics

Gene Symbol MTHFD2