MTF2 Antibody

MTF2 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human MTF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6846) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

MTF2 Antibody Summary

• Immunogen: E. coli-derived recombinant human MTF2
  Asn356-Ser554
  Accession # Q9Y483
• Specificity: Detects human MTF2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: MTF2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MTF2 Antibody

• M96
• metal response element binding transcription factor 2
• MTF2
• PCI2
• PCL2

Background

MTF2 (Metal-response element-binding transcription factor 2; also M96, ZiRF1 and PCL2) is a 67 kDa member of the polycomb group of proteins. It appears to participate in the activities of at least two complexes, PRC1 (Polycomb repressive complex 1) and PRC2. In general, the PRC complexes are repressive. In stem cells, MTF2/PRC2 inhibits expression of pluripotency factors such as KLF4 and Tbx3, likely by recruiting the full PRC2 complex to target gene promoters. And during AP specification of the axis, MTF2, as part of both a PRC1 and PRC2 complex, blocks Hox gene expression. Human MTF2 is 593 amino acids (aa) in length. It contains one Tudor domain (aa 40-91), two PHD-type Zn-finger regions (aa 102-157 and 201-255) and three utilized phosphorylation sites at Ser24, 27 and 488. There are multiple potential splice variants. One is 60 kDa in size and shows a deletion of aa 331-387, while a second is 55 kDa in size and possesses an alternative start site at Met103. A third contains a combination of the above, a fourth possesses a 34 aa substitution for aa 1-307, and a fifth utilizes an alternative start site at Met332. Over aa 356-554, human MTF2 shares 94% aa identity with mouse MTF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTF2
• Uniprot:
  • Q9Y483(Human)