MSPR/Ron Antibody

Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific) . Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND50 is typically 0.05-0.2 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MSPR/Ron Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MSP R/Ron
  Ser24-Arg960 (Ile372Leu)
  Accession # CAA52754
• Specificity: Detects mouse MSP R/Ron in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human MSP R/Ron is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MST1R
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.05-0.2 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSPR/Ron Antibody

• CD136 antigen
• CD136
• CDw136c-met-related tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• MSP R
• MSP receptor
• MSPR
• MST1R
• p185-Ron
• Protein-tyrosine kinase 8
• PTK8 protein tyrosine kinase 8
• PTK8
• Ron
• RONmacrophage-stimulating protein receptor
• soluble RON variant 1
• soluble RON variant 2
• soluble RON variant 3
• soluble RON variant 4

Background

Macrophage stimulating protein receptor (MSP R), encoded by the human RON and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea. This family of receptors is synthesized as a single-chain precursor that is cleaved into a mature disulfide-linked heterodimer composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Mouse MSP R cDNA encodes a 1378 amino acid (aa) residue presursor protein with a 23 aa signal peptide, a 287 aa residue alpha chain (Ser 24 - Arg 310) and a 1068 aa residue transmembrane beta chain (Gly 311 - Thr 1378). Expression of MSP receptor is restricted to specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage. Both the heterodimeric MSP and the free MSP beta chain have been shown to bind to MSP R. However, only the heterodimeric MSP binding can induce receptor dimerization and phosphorylation and cause biological activity.

1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
2. Wang, M-H. et al. (1994) Science 266:117.
3. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSPR/Ron Antibody (AF431)(3)

Publications using AF431

• A Dey, JN Allen, JW Fraser, LM Snyder, Y Tian, L Zhang, RF Paulson, A Patterson, MT Cantorna, PA Hankey-Gib Neuroprotective Role of the Ron Receptor Tyrosine Kinase Underlying Central Nervous System Inflammation in Health and Disease Front Immunol, 2018-03-19;9(0):513. 2018-03-19 [PMID: 29616029] (Flow Cytometry, Mouse)
• Kauder , Steven E, Santell , Lydia, Mai , Elaine, Wright , Lilyan Y, Luis , Elizabet, N'Diaye , Elsa N, Lutman , Jeff, Ratti , Navneet, Sa , Susan M, Maun , Henry R, Stefanich , Eric, Gonzalez , Lino C, Graham , Robert R, Diehl , Lauri, Faubion , William, Keir , Mary E, Young , Judy, Chaudhuri , Amitabha, Lazarus , Robert A, Egen , Jackson Functional consequences of the macrophage stimulating protein 689C inflammatory bowel disease risk allele. PLoS ONE, 2013-12-23;8(12):e83958. 2013-12-23 [PMID: 24409221] (IHC-P, Mouse)
• William D. Stuart, Rishikesh M. Kulkarni, Jerilyn K. Gray, Juozas Vasiliauskas, Mike A. Leonis, Susan E. Waltz The Ron Receptor Regulates Kupffer Cell-Dependent Cytokine Production and Hepatocyte Survival Following Endotoxin Exposure in Mice Hepatology 5/1/2011 [PMID: 21520175]

Bioinformatics

• Gene Symbol: MST1R
• Uniprot:
  • CAA52754()