MST1R Products By Gene Name

HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947)." class="big_lightbox"> 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947)." class="big_thumb" />

352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MSP R/Ron at approximately 150-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

352-MS) for 10 minutes. MSP R/Ron was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691). The MSP R/Ron-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron was detected by Western blot using 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691)." class="big_lightbox"> 352-MS) for 10 minutes. MSP R/Ron was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691). The MSP R/Ron-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron was detected by Western blot using 0.2 µg/mL of Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF691)." class="big_thumb" />

352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific) . Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND₅₀ is typically 0.05-0.2 µg/mL." class="big_lightbox"> 352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific) . Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND₅₀ is typically 0.05-0.2 µg/mL." alt="Recombinant Human MSP (Catalog # 352-MS) chemoattracts mouse peritoneal macrophages in a dose-dependent manner (orange line). The amount of cells that migrated through filter was measured by LeukoStat™ staining (Fisher Scientific) . Chemotaxis elicited by Recombinant Human MSP (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse MSP R/Ron Antigen Affinity-purified Polyclonal Antibody (Catalog # AF431). The ND₅₀ is typically 0.05-0.2 µg/mL." class="big_thumb" />

352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot." class="big_lightbox"> 352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot." alt="MDA-MB-453 human breast cancer cells were treated with 400 ng/mL recombinant human MSP (Catalog # 352-MS) for five minutes to induce tyrosine phosphorylation of MSP R. Serial dilutions of lysates were analyzed by (A) IP-Western Blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-MSP R monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-MSP R. Bands were visualized with Streptavidin-HRP (Catalog # DY998) followed by chemiluminescent detection. Human phospho-MSP R can be detected in this DuoSet® IC ELISA by using approximately 6-12 times less lysate than is needed for a conventional IP-Western Blot." class="big_thumb" />

BAF691)." class="big_lightbox"> BAF691)." class="big_thumb" />

358-MT), human IGF-I sR (Catalog # 391-GR), or human EGF R (Catalog # 1095-ER) were added to 100 μg lysate and analyzed using this DuoSet® IC ELISA. Competition was observed only with recombinant human MSP R." class="big_lightbox"> 358-MT), human IGF-I sR (Catalog # 391-GR), or human EGF R (Catalog # 1095-ER) were added to 100 μg lysate and analyzed using this DuoSet® IC ELISA. Competition was observed only with recombinant human MSP R." class="big_thumb" />