MSH2 Antibody (CL13713) - BSA Free

Western blot analysis in human cell line NTERA-2.

Analysis in human testis and liver tissues using NBP3-24572 antibody. Corresponding MSH2 RNA-seq data are presented for the same tissues.

Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.

Staining in NTERA-2 cell line with Anti-MSH2 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Staining of human small intestine shows strong nuclear positivity in glandular cells.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: CL13713
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MSH2 Antibody (CL13713) - BSA Free Summary

• Description: Novus Biologicals Mouse MSH2 Antibody (CL13713) - BSA Free (NBP3-24572) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P43246, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (95%), Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MSH2 Antibody (CL13713) - BSA Free

• COCA1
• DNA mismatch repair protein Msh2
• FCC1
• hMSH2
• HNPCC
• HNPCC1
• HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
• LCFS2
• MSH2
• mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH2