MRPS2 Antibody - BSA Free

Western Blot: MRPS2 Antibody [NBP3-06045] - Anti-MRPS2 rabbit polyclonal antibody at 1:500 dilution Lane A: A-431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:33 kDa

Immunohistochemistry-Paraffin: MRPS2 Antibody [NBP3-06045] - Immunochemical staining of human MRPS2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: MRPS2 Antibody [NBP3-06045] - MRPS2 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-MRPS2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-MRPS2 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa Observed band size :33 kDa

Immunocytochemistry/Immunofluorescence: MRPS2 Antibody [NBP3-06045] - Immunofluorescence staining of MRPS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06045 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPS2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MRPS2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPS2 Antibody - BSA Free

• 28S ribosomal protein S2, mitochondrial
• CGI-91
• mitochondrial 28S ribosomal protein S2
• mitochondrial ribosomal protein S2
• MRP-S2
• S2mt

Background

The Mitochondrial Ribosomal Protein S2, or MRPS2, consists of a 296 long amino acid isoform that is 33 kDa, and is involved in protein synthesis as a component of the 28S subunit on the mitochondrial ribosome. Disease research is currently being conducted on the relationship between MRPS2 and pneumonia and mycobacterium tuberculosis. MRPS2 has been linked to the process of translation, and interacts with MRPS5, ICT1, ESR2, MRPS14, and MRPS11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPS2