BTN3A3 Antibody - BSA Free

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining in human spleen and skeletal muscle tissues using NBP1-88509 antibody. Corresponding BTN3A3 RNA-seq data are presented for the same tissues.

Western Blot: BTN3A3 Antibody [NBP1-88509] - Analysis in human cell line Jurkat.

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human lymph node shows strong membranous positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human rectum shows strong membranous positivity in lymphoid cells.

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Immunohistochemistry-Paraffin: BTN3A3 Antibody [NBP1-88509] - Staining of human spleen shows strong membranous positivity in cells in red pulp.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BTN3A3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: SHIYTFPHASFSEPLYPVFRILTLEPTALTICPIPKEVESSPDPDLVPDHSLETPLTPGLANESGEPQAEVTSLLLPAHPGAEVSPSATTNQNHKLQARTEAL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BTN3A3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  BTN3A3 Protein (NBP1-88509PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BTN3A3 Antibody - BSA Free

• BTF3
• BTF3butyrophilin 3
• BTN3.3
• BTN3A3
• butyrophilin subfamily 3 member A3
• butyrophilin, subfamily 3, member A3

Background

BTN3A3 belongs to the BTN3 subfamily of the butyrophilin (BTN) family. BTN genes are MHC-associated and code for membrane proteins. BTN3A1, BTN3A2 and BTN3A3 exhibit very high homology, but only BTN3A1 and BTN3A3 have an intracellular B30.2 (SPRY) domain in addition to the two extracellular Ig domains. BTN3 (CD277) molecules are found on various immune cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTN3A3 Antibody (NBP1-88509)(2)

Publications using NBP1-88509

• Gelman BB, Chen T, Lisinicchia JG et al. The National NeuroAIDS Tissue Consortium brain gene array: two types of HIV-associated neurocognitive impairment. PLoS One 2012-01-01 [PMID: 23049970]
• Le Page C, Marineau A, Bonza PK et al. BTN3A2 expression in epithelial ovarian cancer is associated with higher tumor infiltrating T cells and a better prognosis. PLoS One 2012-01-01 [PMID: 22685580]

Bioinformatics

• Gene Symbol: BTN3A3