MRP3 Antibody (M3II-21)

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clone: M3II-21
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MRP3 Antibody (M3II-21) Summary

• Description: Novus Biologicals Mouse MRP3 Antibody (M3II-21) (NBP1-42348) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-MRP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: MRP3 (aa 830-949)
• Specificity: Reacts with an internal epitope of MRP3. Does not cross-react with the human MDR1, MRP1, MRP2 or MRP5 gene products
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCC3
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Frozen 1:20
  • Simple Western
  • Western Blot 1:20-1:50
• Application Notes: M3II-21 has potential value for detection of MRP3-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-1:50 dil. on acetone or formaldehyde fixed cytospin preparations. For immunohistochemistry: use 1:20 on acetone fixed frozen sections. Western Blot: use 1:20-1:50 dil, and anti-Mouse HRP. Flow Cytometry: optimal conditions still to be defined. Use in Simple Western reported in scientific literature (PMID: 29890265).
  See Simple Western Antibody Database for Simple Western validation: separated by Size

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Supernatant, 0.7% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MRP3 Antibody (M3II-21)

• ABC31
• ABCC3
• ATP-binding cassette sub-family C member 3
• ATP-binding cassette, sub-family C (CFTR/MRP), member 3
• canalicular multispecific organic anion transporter 2
• canicular multispecific organic anion transporter
• CMOAT2
• EC 3.6.3
• EC 3.6.3.44
• EC 6.3.2.4
• EC 7.6.2.3
• MLP2
• MLP2DKFZp686E22157
• MOAT-D
• MRP3
• MRP3MOAT-DEST90757
• multidrug resistance associated protein
• Multidrug resistance-associated protein 3
• Multi-specific organic anion transporter D

Background

The protein encoded by the MRP3 gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. The specific function of this protein has not yet been determined; however, this protein may play a role in the transport of biliary and intestinal excretion of organic anions. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRP3 Antibody (NBP1-42348)(1)

Publication using NBP1-42348

• Mossoba ME, Vohra S, Toomer H et al. Diglycolic acid induces HepG2/C3A liver cell toxicity in vitro Toxicol In Vitro. [PMID: 29890265] (Simple Western, Human)

Bioinformatics

• Gene Symbol: ABCC3