MRGPRF Antibody - BSA Free

Immunohistochemistry-Paraffin: MRGPRF Antibody [NBP3-14410] - MRGPRF antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

• Reactivity: Hu, Bv, Ca, Pm
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MRGPRF Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 18 amino acid peptide from N-Terminus of human MRGPRF.
• Epitope: N-Terminus
• Specificity: Human MRGPRF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (94%), Canine (94%), Bovine (94%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRGPRF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml..

Reactivity Notes

Expected reactivity based on percent sequence identity: Mouse, Rat, Hamster, Porcine (88%)

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRGPRF Antibody - BSA Free

• FLJ16111
• FLJ29034
• FLJ40998
• FLJ53714
• G protein-coupled receptor 140
• G protein-coupled receptor 168
• G protein-coupled receptor MrgF
• GPR140
• GPR168
• G-protein coupled receptor 140
• G-protein coupled receptor 168
• mas-related G protein-coupled MRGF
• Mas-related gene F protein
• MAS-related GPR, member F
• mas-related G-protein coupled receptor member F
• MGC21621
• mrgF
• MRGFDKFZp586B2122
• MRGPRF
• RTA
• seven transmembrane helix receptor

Background

MRGF is an orphan receptor that may bind to a neuropeptide and may regulate nociceptor function and/or development, including the sensation or modulation of pain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRGPRF