KSHV ORF57 Antibody

Western Blot: KSHV ORF57 Antibody [NBP2-95260] - Western blot using KSHV ORF57 Antibody to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow). The membrane was probed with the primary antibody diluted 1:7,500 (left) and 1:1,000 (right).

• Reactivity: V-Vi
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

KSHV ORF57 Antibody Summary

• Immunogen: This affinity purified KSHV ORF57 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human KSHV ORF57 protein. (Uniprot: Q2HR75)
• Localization: Tumor progenitor endothelial cells
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100000-170000
  • Immunohistochemistry
  • Western Blot 1:5000-1:8000
• Application Notes: Specific conditions for reactivity should be optimized by the end user. Expect a band 50-55 kDa in size corresponding to KSHV KSHV ORF57 protein by western blotting in the appropriate cell lysate or extract.
• Theoretical MW: 51.01 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with KSHV ORF57 protein from human herpesvirus 8 types P and M sources based on 94% homology with the immunizing sequence. Reactivity against homologues from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human herpesvirus 8 (KSHV ORF57) protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography

Alternate Names for KSHV ORF57 Antibody

• herpesvirus core gene UL54 family
• ORF57

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.