MRCL3 [p Ser19, p Ser20] Antibody

Images

 
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is ...read more
Immunohistochemistry: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MRCL3 [p Ser19, p Ser20] Antibody Summary

Immunogen
Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: P19105)
Modification
p Ser19, p Ser20
Localization
Cytoplasmic
Specificity
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific for the phosphorylated [p Ser19, p Ser20] form of the protein, depending on the source origin of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYL12A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:30000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
Application Notes
This antibody was tested by ELISA, immunohistochemistry and western blotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes, 3T3 whole cell lysates, and regulatory light chain and smooth muscle phospho recombinant protein. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation.
Theoretical MW
19.79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1687 in the following applications:

Reactivity Notes

Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for MRCL3 [p Ser19, p Ser20] Antibody

  • MLCBMyosin RLC
  • MRCL3
  • MRLC3MLC-2B
  • MYL2B
  • myosin regulatory light chain 12A
  • Myosin regulatory light chain 2, nonsarcomeric
  • myosin regulatory light chain 3
  • myosin regulatory light chain MRCL3
  • Myosin regulatory light chain MRLC3
  • myosin, light chain 12A, regulatory, non-sarcomeric
  • myosin, light polypeptide, regulatory, non-sarcomeric (20kD)
  • RLC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67486
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB4470
Species: All-Multi
Applications: ICC, IHC, WB
H00085366-M01
Species: Hu, Mu
Applications: ELISA, KD, WB
NBP2-62682
Species: Hu
Applications: IHC, IHC-P
361-MI/CF
Species: Hu
Applications: BA
NBP2-38323
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-85541
Species: Hu, Mu
Applications: IHC, IHC-Fr, IHC-P, WB
NBP2-14871
Species: Mu, Rt
Applications: ICC/IF, WB
NB120-2860
Species: Ba, Bv, Ch, Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-15669
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-62625
Species: Hu
Applications: IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NBP2-54719
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-06985
Species: Hu
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
NB100-624
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-78159
Species: Bv, Hu, Po, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA, WB
NB100-1687
Species: Hu, Mu
Applications: WB, ELISA, IHC, IHC-P, IP

Publications for MRCL3 Antibody (NB100-1687) (0)

There are no publications for MRCL3 Antibody (NB100-1687).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for MRCL3 Antibody (NB100-1687) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-1687:
Filter by Applications
Western Blot
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MRCL3 NB100-1687
Enlarge
5
reviewed by:
L P
Western Blot Mouse 05/30/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse EDL skeletal muscle
SpeciesMouse

Comments

Comments This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MRCL3 Antibody (NB100-1687) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MRCL3 Products

Bioinformatics Tool for MRCL3 Antibody (NB100-1687)

Discover related pathways, diseases and genes to MRCL3 Antibody (NB100-1687). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MRCL3 Antibody (NB100-1687)

Discover more about diseases related to MRCL3 Antibody (NB100-1687).
 

Pathways for MRCL3 Antibody (NB100-1687)

View related products by pathway.

PTMs for MRCL3 Antibody (NB100-1687)

Learn more about PTMs related to MRCL3 Antibody (NB100-1687).
 

Research Areas for MRCL3 Antibody (NB100-1687)

Find related products by research area.

Blogs on MRCL3

There are no specific blogs for MRCL3, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

L P
05/30/2017
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol MYL12A
Entrez