Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is ...read more
Immunohistochemistry: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Affinity purified phosphospecific antibody to monophosphorylated regulatory light chain of smooth and non-muscle Myosin at pS19 was used at a 1:1000 ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 l of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle ...read more
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate ...read more
Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: P19105)
p Ser19, p Ser20
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. This phosphospecific polyclonal antibody is specific for the phosphorylated [p Ser19, p Ser20] form of the protein, depending on the source origin of the protein. Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA.
Immunogen affinity purified
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This antibody was tested by ELISA, immunohistochemistry and western blotting. Immunoblotting was used to show reactivity with unstimulated and stimulated cardiac myocytes, 3T3 whole cell lysates, and regulatory light chain and smooth muscle phospho recombinant protein. The antibody was also reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional by immunoprecipitation.
19.79 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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Reactivity with the protein from other species has not been determined. However, the sequence of the immunogen is nearly identical in mammalian and avian species. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences
Packaging, Storage & Formulations
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% Sodium Azide
Immunogen affinity purified
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Alternate Names for MRCL3 [p Ser19, p Ser20] Antibody
This antibody has been shown to cross-react with mouse skeletal muscle regulatory light chain (Lai et al., 2016. Am J Physiol Endocrinol Metab), and is shown to positively react with mouse EDL muscle here.
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