MP1/MAP2K1IP1 Antibody

Images

 
Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MP1/MAP2K1IP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MP1/MAP2K1IP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.
Simple Western: MP1/MAP2K1IP1 Antibody [NBP1-50631] - Simple Western lane view shows a specific band for MP1/MAP2K1IP1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
1.08 mg/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

MP1/MAP2K1IP1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
Modification
No Modifications
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
Clonality
Polyclonal
Host
Rabbit
Gene
LAMTOR3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:40
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommendedIn Simple Western only 10-15 uL of the recommended dilution is used per data point.

Reactivity Notes

Human and mouse.

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MP1/MAP2K1IP1 Antibody

  • LAMTOR3
  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
  • MAP2K1IP1
  • Mapbp
  • MAPK scaffold protein 1
  • MAPKSP1
  • MEK binding partner 1
  • MEK partner 1
  • MEK-binding partner 1
  • mitogen-activated protein kinase kinase 1 interacting protein 1
  • Mitogen-activated protein kinase kinase 1-interacting protein 1
  • Mitogen-activated protein kinase scaffold protein 1
  • MP1
  • PRO0633
  • Ragulator3

Background

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MP1/MAP2K1IP1 Antibody (NBP1-50631) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-50631AF405
Alexa Fluor (R) 488 Labeled NBP1-50631AF488
Alexa Fluor (R) 647 Labeled NBP1-50631AF647
Alexa Fluor (R) 700 Labeled NBP1-50631AF700
Biotin Labeled NBP1-50631B
DyLight 405 Labeled NBP1-50631V
DyLight 488 Labeled NBP1-50631G
DyLight 550 Labeled NBP1-50631R
DyLight 650 Labeled NBP1-50631C
FITC Labeled NBP1-50631F
HRP Labeled NBP1-50631H

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Blogs on MP1/MAP2K1IP1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol LAMTOR3
Entrez
Uniprot

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