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MP1/MAP2K1IP1 Antibody

 
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Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MP1/MAP2K1IP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MP1/MAP2K1IP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.
Simple Western: MP1/MAP2K1IP1 Antibody [NBP1-50631] - Simple Western lane view shows a specific band for MP1/MAP2K1IP1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-50631AF405
Alexa Fluor 488
NBP1-50631AF488
Alexa Fluor 647
NBP1-50631AF647
Alexa Fluor 700
NBP1-50631AF700
Biotin
NBP1-50631B
DyLight 405
NBP1-50631V
DyLight 488
NBP1-50631G
DyLight 550
NBP1-50631R
DyLight 650
NBP1-50631C
FITC
NBP1-50631F
HRP
NBP1-50631H
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MP1/MAP2K1IP1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMTOR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Simple Western 1:40
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
A-431 Lysate (NB820-59461)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MP1/MAP2K1IP1 Antibody

  • LAMTOR3
  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
  • MAP2K1IP1
  • Mapbp
  • MAPK scaffold protein 1
  • MAPKSP1
  • MEK binding partner 1
  • MEK partner 1
  • MEK-binding partner 1
  • mitogen-activated protein kinase kinase 1 interacting protein 1
  • Mitogen-activated protein kinase kinase 1-interacting protein 1
  • Mitogen-activated protein kinase scaffold protein 1
  • MP1
  • PRO0633
  • Ragulator3

Background

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol
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Positive Control Lysate(s)

A-431 Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-50631AF405
Alexa Fluor 488 Labeled NBP1-50631AF488
Alexa Fluor 647 Labeled NBP1-50631AF647
Alexa Fluor 700 Labeled NBP1-50631AF700
Biotin Labeled NBP1-50631B
DyLight 405 Labeled NBP1-50631V
DyLight 488 Labeled NBP1-50631G
DyLight 550 Labeled NBP1-50631R
DyLight 650 Labeled NBP1-50631C
FITC Labeled NBP1-50631F
HRP Labeled NBP1-50631H
Learn about Custom Antibody Labeling

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Diseases for MP1/MAP2K1IP1 Antibody (NBP1-50631)

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  Western Blot Protocol
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Bioinformatics

Gene Symbol LAMTOR3
Entrez
Uniprot