MP1/MAP2K1IP1 Antibody (NBP1-50631)

Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.

Immunocytochemistry/Immunofluorescence: MP1/MAP2K1IP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: MP1/MAP2K1IP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.

Simple Western: MP1/MAP2K1IP1 Antibody [NBP1-50631] - Simple Western lane view shows a specific band for MP1/MAP2K1IP1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.08 mg/ml

MP1/MAP2K1IP1 Antibody Summary

• Immunogen: A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
• Localization: Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LAMTOR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
  • Simple Western 1:40
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Positive Control:

  A-431 Lysate (NB820-59461)

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.08 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MP1/MAP2K1IP1 Antibody

• LAMTOR3
• Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3
• late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
• MAP2K1IP1
• Mapbp
• MAPK scaffold protein 1
• MAPKSP1
• MEK binding partner 1
• MEK partner 1
• MEK-binding partner 1
• mitogen-activated protein kinase kinase 1 interacting protein 1
• Mitogen-activated protein kinase kinase 1-interacting protein 1
• Mitogen-activated protein kinase scaffold protein 1
• MP1
• PRO0633
• Ragulator3

Background

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LAMTOR3
• Entrez:
  • 8649(Human)
  • 56692(Mouse)
  • 362045(Rat)
• Uniprot:
  • Q9UHA4()
  • O88653(Mouse)