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MP1/MAP2K1IP1 Antibody

 
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Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Western Blot: MP1/MAP2K1IP1 Antibody [NBP1-50631] - WB detection of MAP2K1IP1 in A431 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: MP1/MAP2K1IP1 Antibody [NBP1-50631] - ICC staining of MAP2K1IP1 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MP1/MAP2K1IP1 Antibody [NBP1-50631] - IHC staining of MAP2K1IP1 in mouse lung.
Simple Western: MP1/MAP2K1IP1 Antibody [NBP1-50631] - Simple Western lane view shows a specific band for MP1/MAP2K1IP1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.08 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-50631AF350
Alexa Fluor 405
NBP1-50631AF405
Alexa Fluor 488
NBP1-50631AF488
Alexa Fluor 532
NBP1-50631AF532
Alexa Fluor 594
NBP1-50631AF594
Alexa Fluor 647
NBP1-50631AF647
Alexa Fluor 700
NBP1-50631AF700
Alexa Fluor 750
NBP1-50631AF750
Biotin
NBP1-50631B
DyLight 350
NBP1-50631UV
DyLight 405
NBP1-50631V
DyLight 488
NBP1-50631G
DyLight 550
NBP1-50631R
DyLight 594
NBP1-50631DL594
DyLight 650
NBP1-50631C
DyLight 680
NBP1-50631FR
DyLight 755
NBP1-50631IR
FITC
NBP1-50631F
HRP
NBP1-50631H
Janelia Fluor 549
NBP1-50631JF549
Janelia Fluor 646
NBP1-50631JF646
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MP1/MAP2K1IP1 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human MAP2K1IP1/MAPKSP1 protein (within residues 20-180). [Swiss-Prot Q9UHA4]
Localization
Late endosome membrane; Peripheral membrane protein; Cytoplasmic side
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMTOR3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:40
  • Western Blot 1:2000
Application Notes
This MAP2K1IP1 antibody is useful for ICC/IF, IHC and Western blot where a band is seen ~13 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
A-431 Whole Cell Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.08 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MP1/MAP2K1IP1 Antibody

  • LAMTOR3
  • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3
  • late endosomal/lysosomal adaptor, MAPK and MTOR activator 3
  • MAP2K1IP1
  • Mapbp
  • MAPK scaffold protein 1
  • MAPKSP1
  • MEK binding partner 1
  • MEK partner 1
  • MEK-binding partner 1
  • mitogen-activated protein kinase kinase 1 interacting protein 1
  • Mitogen-activated protein kinase kinase 1-interacting protein 1
  • Mitogen-activated protein kinase scaffold protein 1
  • MP1
  • PRO0633
  • Ragulator3

Background

Due to its ability to enhance activation of MAPK2, Mapksp1 seems to enhance the efficiency of the MAP kinase cascade. It is known to bind specifically to MAP2K1/MEK1, MAPK3/ERK1, and MAPK1/ERK2. The increased efficiency of the MAP cascade directly affects the regulation of the late endosomal traffic and cell proliferation in endosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of human hippocampal and rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Human FABP8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5866) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP8 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
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NBP1-87431
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AF2065
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MP1/MAP2K1IP1
Antibody
NBP1-50631
MP1/MAP2K1IP1 Antibody
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

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Product General Protocols

View specific protocols for MP1/MAP2K1IP1 Antibody (NBP1-50631): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

A-431 Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-50631AF350
Alexa Fluor 405 NBP1-50631AF405
Alexa Fluor 488 NBP1-50631AF488
Alexa Fluor 532 NBP1-50631AF532
Alexa Fluor 594 NBP1-50631AF594
Alexa Fluor 647 NBP1-50631AF647
Alexa Fluor 700 NBP1-50631AF700
Alexa Fluor 750 NBP1-50631AF750
Biotin NBP1-50631B
DyLight 350 NBP1-50631UV
DyLight 405 NBP1-50631V
DyLight 488 NBP1-50631G
DyLight 550 NBP1-50631R
DyLight 594 NBP1-50631DL594
DyLight 650 NBP1-50631C
DyLight 680 NBP1-50631FR
DyLight 755 NBP1-50631IR
FITC NBP1-50631F
HRP NBP1-50631H
Janelia Fluor 549 NBP1-50631JF549
Janelia Fluor 646 NBP1-50631JF646
Learn about Custom Antibody Labeling

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Diseases for MP1/MAP2K1IP1 Antibody (NBP1-50631)

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Product PDFs

  ICC/IF Protocol
  IHC-P Protocol
  Western Blot Protocol
  SDS
  PDF Datasheet
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Bioinformatics

Gene Symbol LAMTOR3
Entrez
Uniprot