Mouse VEGFR1/Flt-1 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse VEGFR1/Flt-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R...1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.
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• Specificity: Natural and recombinant mouse sVEGF R1
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Flt1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit

• EC 2.7.10
• EC 2.7.10.1
• FLT
• FLT1
• Flt-1
• Fms-like tyrosine kinase 1
• fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
• FRT
• Tyrosine-protein kinase FRT
• Tyrosine-protein kinase receptor FLT
• vascular endothelial growth factor receptor 1
• Vascular permeability factor receptor
• VEGF R1
• VEGFR1
• VEGFR-1

Background

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGFR1/Flt-1 (MVR100)(39)

Publications using MVR100

• Granitzer, S;Widhalm, R;Ellinger, I;Zeisler, H;Forsthuber, M;Foessleitner, P;Geschrey, E;Saleh, L;Knöfler, M;Desoye, G;Ettel, P;Weichhart, T;Musiejovsky, L;Schabbauer, G;Salzer, H;Rosner, M;Hengstschläger, M;Gundacker, C; LAT1-NRF2 axis controls sFlt-1/PlGF imbalance and oxidative stress in preeclampsia Nature communications 2025-10-14 [PMID: 41087351] (Mouse)
• Alhasan, MM;Landa, MS;García, SI;Gerlach, RG;Harb, H;Fahlbusch, FB;Conrad, ML;Barrientos, G; Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia Scientific reports 2024-12-30 [PMID: 39738527] (Mouse)
• Yang, Z;Jia, X;Deng, Q;Luo, M;Hou, Y;Yue, J;Mei, J;Shan, N;Wu, Z; Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/?-catenin signaling to alleviate preeclampsia International immunopharmacology 2023-02-01 [PMID: 37724958] (Mouse)
• X Liu, Z Li, J Sun, Z Zhang, W Li Interaction between PD-L1 and soluble VEGFR1 in glioblastoma-educated macrophages BMC Cancer, 2023-03-20;23(1):259. 2023-03-20 [PMID: 36941554] (Human)
• SL Lu, H Omori, Y Zhou, YS Lin, CC Liu, JJ Wu, T Noda VEGF-Mediated Augmentation of Autophagic and Lysosomal Activity in Endothelial Cells Defends against Intracellular Streptococcus pyogenes MBio, 2022-07-05;0(0):e0123322. 2022-07-05 [PMID: 35862783] (Mouse)
• M Jones-Muha, Q Shao, L Cain-Shiel, JP Shaffery, JP Warrington Acid Sensing Ion Channel 2a Is Reduced in the Reduced Uterine Perfusion Pressure Mouse Model and Increases Seizure Susceptibility in Pregnant Mice Cells, 2021-05-08;10(5):. 2021-05-08 [PMID: 34066660] (Mouse)
• YY Shin, SM An, JS Jeong, SY Yang, GS Lee, EJ Hong, EB Jeung, SC Kim, BS An Comparison of steroid hormones in�three�different preeclamptic models Molecular Medicine Reports, 2021-02-04;23(4):. 2021-02-04 [PMID: 33537808] (Human, Rat)
• JG Parchem, K Kanasaki, SB Lee, M Kanasaki, JL Yang, Y Xu, KM Earl, RA Keuls, VH Gattone, R Kalluri STOX1 deficiency is associated with renin-mediated gestational hypertension and placental defects JCI Insight, 2021-01-25;0(0):. 2021-01-25 [PMID: 33301424] (Mouse)
• DJ Dijkstra, RN Verkaik-Sc, S Eskandar, A Limonciel, V Stojanovsk, SA Scherjon, T Plösch Mid-gestation low-dose LPS administration results in female-specific excessive weight gain upon a western style diet in mouse offspring Sci Rep, 2020-11-12;10(1):19618. 2020-11-12 [PMID: 33184349] (Mouse)
• L Erlandsson, A Ducat, J Castille, I Zia, G Kalapothar, E Hedström, JL Vilotte, D Vaiman, SR Hansson Alpha-1 microglobulin as a potential therapeutic candidate for treatment of hypertension and oxidative stress in the STOX1 preeclampsia mouse model Sci Rep, 2019-06-12;9(1):8561. 2019-06-12 [PMID: 31189914] (Mouse)
• MW Cunningham, J Castillo, T Ibrahim, DC Cornelius, N Campbell, L Amaral, VR Vaka, N Usry, JM Williams, B LaMarca AT1-AA (Angiotensin II Type 1 Receptor Agonistic Autoantibody) Blockade Prevents Preeclamptic Symptoms in Placental Ischemic Rats Hypertension, 2018-03-19;0(0):. 2018-03-19 [PMID: 29555668] (Rat)
• KD Beharry, CL Cai, T Ahmad, S Guzel, GB Valencia, JV Aranda Impact of Chronic Neonatal Intermittent Hypoxia on Severity of Retinal Damage in a Rat Model of Oxygen-Induced Retinopathy J Nat Sci, 2018-01-01;4(3):. 2018-01-01 [PMID: 29552637] (Rat)
• D Reijnders, CC Liu, X Xu, A Zhao, K Olson, SD Butler, NC Douglas, JL Sones Celecoxib restores angiogenic factor expression at the maternal-fetal interface in the BPH/5 mouse model of preeclampsia Physiol. Genomics, 2018-03-09;0(0):. 2018-03-09 [PMID: 29521599] (ELISA Detection, Mouse)
• SM Blois, N Freitag, I Tirado-Gon, SB Cheng, MM Heimesaat, S Bereswill, M Rose, ML Conrad, G Barrientos, S Sharma NK cell-derived IL-10 is critical for DC-NK cell dialogue at the maternal-fetal interface Sci Rep, 2017-05-19;7(1):2189. 2017-05-19 [PMID: 28526846] (Mouse)
• P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al Motor neurons control blood vessel patterning in the developing spinal cord Nat Commun, 2017-03-06;8(0):14583. 2017-03-06 [PMID: 28262664] (Mouse)
• Luise Erpenbeck PAD4 Deficiency Decreases Inflammation and Susceptibility to Pregnancy Loss in a Mouse Model Biol. Reprod, 2016-11-09;95(6):132. 2016-11-09 [PMID: 28007693] (Mouse)
• J Morgan Denney Renin-Angiotensin System Transgenic Mouse Model Recapitulates Pathophysiology Similar to Human Preeclampsia with Renal Injury that may be Mediated through VEGF Am. J. Physiol. Renal Physiol, 2016-12-07;0(0):ajprenal.0010. 2016-12-07 [PMID: 27927648] (Mouse)
• Reduced Uterine Perfusion Pressure (RUPP) Model of Preeclampsia in Mice PLoS ONE, 2016-05-17;11(5):e0155426. 2016-05-17 [PMID: 27187738] (Mouse)
• Venditti , Carolina, Casselman , Richard, Young , Iain, Karumanchi , S Ananth, Smith , Graeme N Carbon monoxide prevents hypertension and proteinuria in an adenovirus sFlt-1 preeclampsia-like mouse model. PLoS ONE, 2014-09-09;9(9):e106502. 2014-09-09 [PMID: 25202912] (Mouse)
• Ayala de la Pena F, Kanasaki K, Kanasaki M, Vong S, Rovira C, Kalluri R Specific activation of K-RasG12D allele in the bladder urothelium results in lung alveolar and vascular defects. PLoS ONE, 2014-04-23;9(4):e95888. 2014-04-23 [PMID: 24760005] (Mouse)
• Luo Y, Kumar P, Chen C, Latham J, Wang L, Tudela C, Alexander J, Shelton J, McKown L, Mendelson C Estrogen-related receptor gamma serves a role in blood pressure homeostasis during pregnancy. Mol Endocrinol, 2014-04-11;28(6):965-75. 2014-04-11 [PMID: 24725083] (Mouse)
• Craici , Iasmina, Wagner , Steven J, Weissgerber , Tracey L, Grande , Joseph P, Garovic , Vesna D Advances in the pathophysiology of pre-eclampsia and related podocyte injury. Kidney Int, 2014-02-26;86(2):275-85. 2014-02-26 [PMID: 24573315] (Rat)
• Justiniano S, Elavazhagan S, Fatehchand K, Shah P, Mehta P, Roda J, Mo X, Cheney C, Hertlein E, Eubank T, Marsh C, Muthusamy N, Butchar J, Byrd J, Tridandapani S Fcgamma receptor-induced soluble vascular endothelial growth factor receptor-1 (VEGFR-1) production inhibits angiogenesis and enhances efficacy of anti-tumor antibodies. J Biol Chem, 2013-07-31;288(37):26800-9. 2013-07-31 [PMID: 23902770] (Mouse)
• Suryawanshi A, Mulik S, Sharma S Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor. J. Immunol., 2011-02-16;186(6):3653-65. 2011-02-16 [PMID: 21325621] (Mouse)
• Kalkunte S, Boij R, Norris W Sera from preeclampsia patients elicit symptoms of human disease in mice and provide a basis for an in vitro predictive assay. Am. J. Pathol., 2010-10-01;177(5):2387-98. 2010-10-01 [PMID: 20889559] (Mouse)
• Tam Tam KB, Lamarca B, Arany M, Cockrell K, Fournier L, Murphy S, Martin JN, Granger JP Role of reactive oxygen species during hypertension in response to chronic antiangiogenic factor (sFlt-1) excess in pregnant rats. Am. J. Hypertens., 2010-08-19;24(1):110-3. 2010-08-19 [PMID: 20725052] (Rat)
• Mazzone M, Dettori D, Leite de Oliveira R, Loges S, Schmidt T, Jonckx B, Tian YM, Lanahan AA, Pollard P, Ruiz de Almodovar C, De Smet F, Vinckier S, Aragones J, Debackere K, Luttun A, Wyns S, Jordan B, Pisacane A, Gallez B, Lampugnani MG, Dejana E, Simons M, Ratcliffe P, Maxwell P, Carmeliet P Heterozygous deficiency of PHD2 restores tumor oxygenation and inhibits metastasis via endothelial normalization. Cell, 2009-02-12;136(5):839-51. 2009-02-12 [PMID: 19217150] (Mouse)
• Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S (Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system. Am. J. Pathol., 2008-10-30;173(6):1911-8. 2008-10-30 [PMID: 18974301] (Mouse)
• Poesen K, Lambrechts D, Van Damme P, Dhondt J, Bender F, Frank N, Bogaert E, Claes B, Heylen L, Verheyen A, Raes K, Tjwa M, Eriksson U, Shibuya M, Nuydens R, Van Den Bosch L, Meert T, D'Hooge R, Sendtner M, Robberecht W, Carmeliet P Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration. J. Neurosci., 2008-10-15;28(42):10451-9. 2008-10-15 [PMID: 18923022] (Rat)
• Zhou CC, Zhang Y, Irani RA, Zhang H, Mi T, Popek EJ, Hicks MJ, Ramin SM, Kellems RE, Xia Y Angiotensin receptor agonistic autoantibodies induce pre-eclampsia in pregnant mice. Nat. Med., 2008-07-27;14(8):855-62. 2008-07-27 [PMID: 18660815] (Mouse)
• Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin. Invest. Ophthalmol. Vis. Sci., 2008-04-01;49(4):1679-85. 2008-04-01 [PMID: 18385091] (Mouse)
• Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization. FASEB J., 2008-04-01;22(8):2775-83. 2008-04-01 [PMID: 18381816] (Mouse)
• Tsao PN, Chan FT, Wei SC, Hsieh WS, Chou HC, Su YN, Chen CY, Hsu WM, Hsieh FJ, Hsu SM Soluble vascular endothelial growth factor receptor-1 protects mice in sepsis. Crit. Care Med., 2007-08-01;35(8):1955-60. 2007-08-01 [PMID: 17568329] (Mouse)
• Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am. J. Physiol. Heart Circ. Physiol., 2007-03-16;293(1):H474-81. 2007-03-16 [PMID: 17369464] (Rat)
• Hara A, Wada T, Furuichi K, Sakai N, Kawachi H, Shimizu F, Shibuya M, Matsushima K, Yokoyama H, Egashira K, Kaneko S Blockade of VEGF accelerates proteinuria, via decrease in nephrin expression in rat crescentic glomerulonephritis. Kidney Int., 2006-06-01;69(11):1986-95. 2006-06-01 [PMID: 16641924] (Transgenic Rat)
• Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts. Am. J. Physiol. Heart Circ. Physiol., 2006-04-14;291(3):H1290-8. 2006-04-14 [PMID: 16617130] (Rat)
• Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor. Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):1078-84. 2005-03-01 [PMID: 15728568] (Mouse)
• Sako A, Kitayama J, Koyama H, Ueno H, Uchida H, Hamada H, Nagawa H Transduction of soluble Flt-1 gene to peritoneal mesothelial cells can effectively suppress peritoneal metastasis of gastric cancer. Cancer Res., 2004-05-15;64(10):3624-8. 2004-05-15 [PMID: 15150121] (Mouse)
• Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J. Clin. Invest., 2003-03-01;111(5):649-58. 2003-03-01 [PMID: 12618519] (Rat)

FAQs for VEGFR1/Flt-1 (MVR100). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Flt1