Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse Sonic Hedgehog N-Terminus Immunoassay is a 4.5 hour solid phase ELISA designed to measure Shh-N in cell culture supernates, serum, and plasma. It contains recombinant mouse Shh-N and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Shh-N showed dose-resp...onse curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Shh-N.
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• Specificity: Natural and recombinant mouse Shh-N. This kit also recognizes low levels of Shh-N in rat serum.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Shh

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Sonic Hedgehog/Shh N-Terminus Quantikine ELISA Kit

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Sonic Hedgehog/Shh (MSHH00)(5)

Publications using MSHH00

• Courtney, Y;Head, JP;Dani, N;Chechneva, OV;Shipley, FB;Zhang, Y;Holtzman, MJ;Sadegh, C;Libermann, TA;Lehtinen, MK; Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development Nature neuroscience 2025-05-28 [PMID: 40437054] (Mouse)
• Wofford, W;Kim, J;Kim, D;Janneh, AH;Lee, HG;Atilgan, FC;Oleinik, N;Kassir, MF;Saatci, O;Chakraborty, P;Tokat, UM;Gencer, S;Howley, B;Howe, P;Mehrotra, S;Sahin, O;Ogretmen, B; Alterations of ceramide synthesis induce PD-L1 internalization and signaling to regulate tumor metastasis and immunotherapy response Cell reports 2024-07-23 [PMID: 39046874] (Mouse)
• B ?wierczek-, D Dudka, D Bauer, T Czajkowski, K Ilach, W Streminska, A Kominek, K Piwocka, MA Ciemerych, K Archacka Comparison of Differentiation Pattern and WNT/SHH Signaling in Pluripotent Stem Cells Cultured under Different Conditions Cells, 2021-10-14;10(10):. 2021-10-14 [PMID: 34685722] (Mouse)
• ME Coulter, CM Dorobantu, GA Lodewijk, F Delalande, S Cianferani, VS Ganesh, RS Smith, ET Lim, CS Xu, S Pang, ET Wong, HGW Lidov, ML Calicchio, E Yang, DM Gonzalez, TM Schlaeger, GH Mochida, H Hess, WA Lee, MK Lehtinen, T Kirchhause, D Haussler, FMJ Jacobs, R Gaudin, CA Walsh The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles Cell Rep, 2018-07-24;24(4):973-986.e8. 2018-07-24 [PMID: 30044992] (Mouse)
• Lun M, Johnson M, Broadbelt K, Watanabe M, Kang Y, Chau K, Springel M, Malesz A, Sousa A, Pletikos M, Adelita T, Calicchio M, Zhang Y, Holtzman M, Lidov H, Sestan N, Steen H, Monuki E, Lehtinen M Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production. J Neurosci, 2015-03-25;35(12):4903-16. 2015-03-25 [PMID: 25810521] (Mouse)

FAQs for Sonic Hedgehog/Shh (MSHH00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Shh