Mouse RAGE Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse RAGE Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse RAGE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse RAGE levels in cell culture supernates, tissue lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse RAGE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse RAGE accurately. Results obtained using natur...al mouse RAGE showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse RAGE.
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• Specificity: Natural and recombinant mouse RAGE.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ager

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse RAGE Quantikine ELISA Kit

• advanced glycosylation end product-specific receptor
• AGER
• RAGE isoform delta
• RAGE isoform sRAGE-delta
• RAGE
• Receptor for advanced glycosylation end products
• receptor for advanced glycosylation end-products
• SCARJ1

Background

RAGE (Receptor for Advanced Glycation End product) is a transmembrane glycoprotein that binds advanced glycation end products (AGEs), beta-amyloid peptides, HMGB1/Amphoterin, and several S100 family proteins. AGEs are adducts formed by the non-enzymatic glycation and oxidation of proteins and lipids. A soluble form can also be generated by MMP-mediated shedding. RAGE is expressed in the CNS during development as well as in adult endothelial cells, smooth muscle cells, pericytes, monocytes, and neurons. It is locally upregulated in vascular inflammation (e.g. diabetes, atherosclerosis, vascular injury, Alzheimer’s disease). At these sites, RAGE binding to S100A1, EN-RAGE/S100A12, or S100B induces inflammatory immune cell adhesion and infiltration as well as vascular smooth muscle proliferation, neointimal expansion, atherosclerotic plaque development, and transport of A-beta into the cerebrospinal fluid. In cancer, RAGE binding to HMGB1, S100A8, or S100A9 promotes tumor growth and metastasis in addition to inflammatory cell infiltration.

To view our complete solutions for RAGE research, visit bio-techne.com.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for AGER (MRG00)(13)

Publications using MRG00

• Taenaka, H;Wick, KD;Sarma, A;Matsumoto, S;Ghale, R;Fang, X;Maishan, M;Gotts, JE;Langelier, CR;Calfee, CS;Matthay, MA; Biological Effects of Corticosteroids on Pneumococcal Pneumonia in Mice and Humans Research square 2024-02-21 [PMID: 38464245] (Mouse)
• Zhai, R;Lenga Ma Bonda, W;Leclaire, C;Saint-Béat, C;Theilliere, C;Belville, C;Coupet, R;Blondonnet, R;Bouvier, D;Blanchon, L;Sapin, V;Jabaudon, M; Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome Journal of translational medicine 2023-06-18 [PMID: 37331963] (Mouse)
• J Zhao, N Zhen, Q Zhou, J Lou, W Cui, G Zhang, B Tian NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982193] (Mouse)
• F Jessop, B Schwarz, D Scott, LM Roberts, E Bohrnsen, JR Hoidal, CM Bosio Impairing RAGE signaling promotes survival and limits disease pathogenesis following SARS-CoV-2 infection in mice JCI Insight, 2022-01-25;7(2):. 2022-01-25 [PMID: 35076028] (Mouse)
• J Islam, AZ Agista, K Watanabe, T Nochi, H Aso, Y Ohsaki, T Koseki, M Komai, H Shirakawa Fermented rice bran supplementation attenuates chronic colitis-associated extraintestinal manifestations in female C57BL/6N mice The Journal of nutritional biochemistry, 2021-09-10;0(0):108855. 2021-09-10 [PMID: 34517096] (Mouse)
• Q Renfeng, C Shuxiao, G Peixian, L Kun, F Xuedong, Y Hai, W Xuejun, L Gang ADAM10 attenuates the development of abdominal aortic aneurysms in a mouse model Molecular Medicine Reports, 2021-09-07;24(5):. 2021-09-07 [PMID: 34490486] (Mouse)
• F Scavello, F Zeni, G Milano, F Macrì, S Castiglion, E Zuccolo, A Scopece, G Pezone, CC Tedesco, P Nigro, G Degani, E Gambini, F Veglia, L Popolo, G Pompilio, GI Colombo, ME Bianchi, A Raucci Soluble Receptor for Advanced Glycation End-products regulates age-associated Cardiac Fibrosis International journal of biological sciences, 2021-06-11;17(10):2399-2416. 2021-06-11 [PMID: 34326683] (Mouse)
• P Swami, KA O'Connell, S Thiyagaraj, A Crawford, P Patil, P Radhakrish, S Shin, TC Caffrey, J Grunkemeye, T Neville, SW Vetter, MA Hollingswo, E Leclerc Inhibition of the Receptor for Advanced Glycation End Products Enhances the Cytotoxic Effect of Gemcitabine in Murine Pancreatic Tumors Biomolecules, 2021-04-01;11(4):. 2021-04-01 [PMID: 33915939] (Mouse)
• H Ohira, A Tsuruya, D Oikawa, W Nakagawa, R Mamoto, M Hattori, T Waki, S Takahashi, Y Fujioka, T Nakayama Alteration of oxidative-stress and related marker levels in mouse colonic tissues and fecal microbiota structures with chronic ethanol administration: Implications for the pathogenesis of ethanol-related colorectal cancer PLoS ONE, 2021-02-12;16(2):e0246580. 2021-02-12 [PMID: 33577585] (Mouse)
• IM Imtiazul, R Asma, JH Lee, NJ Cho, S Park, HY Song, HW Gil Change of surfactant protein D and A after renal ischemia reperfusion injury PLoS ONE, 2019-12-26;14(12):e0227097. 2019-12-26 [PMID: 31877195] (Mouse)
• R Blondonnet, J Audard, C Belville, G Clairefond, J Lutz, D Bouvier, L Roszyk, C Gross, M Lavergne, M Fournet, L Blanchon, C Vachias, C Damon-Soub, V Sapin, JM Constantin, M Jabaudon RAGE inhibition reduces acute lung injury in mice Sci Rep, 2017-08-03;7(1):7208. 2017-08-03 [PMID: 28775380] (Mouse)
• The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE PLoS ONE, 2016-09-21;11(9):e0153832. 2016-09-21 [PMID: 27655137] (Mouse)
• Mouse RAGE Variant 4 Is a Dominant Membrane Receptor that Does Not Shed to Generate Soluble RAGE PLoS ONE, 2016-09-21;11(9):e0153657. 2016-09-21 [PMID: 27655067] (Human, Mouse)

Bioinformatics

• Gene Symbol: Ager