Mouse Osteopontin DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Osteopontin DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Spp1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Osteopontin DuoSet ELISA, 15 Plate

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Publications for Osteopontin/OPN (DY441)(17)

Publications using DY441

• Doherty, DF;Roets, LE;Dougan, CM;Brown, RR;Hawthorne, IJ;O'Kane, C;Krasnodembskaya, AD;Mall, MA;Taggart, CC;Weldon, S; Mesenchymal stromal cells reduce inflammation and improve lung function in a mouse model of cystic fibrosis lung disease Scientific reports 2024-12-28 [PMID: 39730509] (Mouse, Transgenic Mouse)
• C Rizzello, V Cancila, S Sangaletti, L Botti, C Ratti, M Milani, M Dugo, F Bertoni, C Tripodo, C Chiodoni, MP Colombo Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signaling Molecular Cancer, 2022-12-12;21(1):215. 2022-12-12 [PMID: 36503430] (Mouse, Transgenic Mouse)
• Y Fukushima, K Sakamoto, M Matsuda, Y Yoshikai, H Yagita, D Kitamura, M Chihara, N Minato, M Hattori cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T�cells Cell Reports, 2022-09-20;40(12):111373. 2022-09-20 [PMID: 36130493] (Mouse)
• FZ Khamissi, L Ning, E Kefaloyian, H Dun, A Arthanaris, A Keller, JJ Atkinson, W Li, B Wong, S Dietmann, K Lavine, D Kreisel, A Herrlich Identification of kidney injury released circulating osteopontin as causal agent of respiratory failure Science Advances, 2022-02-25;8(8):eabm5900. 2022-02-25 [PMID: 35213222] (Mouse)
• MD Ihrie, KS Duke, KA Shipkowski, DJ You, HY Lee, AJ Taylor-Jus, JC Bonner STAT6-Dependent Exacerbation of House Dust Mite-Induced Allergic Airway Disease in Mice by Multi-Walled Carbon Nanotubes NanoImpact, 2021-03-13;22(0):. 2021-03-13 [PMID: 33860111] (Mouse)
• CC Hu, CH Chang, YM Hsiao, Y Chang, YY Wu, SWN Ueng, MF Chen Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral Defects Int J Mol Sci, 2020-08-03;21(15):. 2020-08-03 [PMID: 32756396] (Mouse)
• S Yoshida, H Nakagami, H Hayashi, Y Ikeda, J Sun, A Tenma, H Tomioka, T Kawano, M Shimamura, R Morishita, H Rakugi The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in mice Nat Commun, 2020-05-18;11(1):2482. 2020-05-18 [PMID: 32424156] (Mouse)
• T Ishii, M Ruiz-Torru, A Ikeda, S Shindo, A Movila, H Mawardi, A Albassam, RA Kayal, AA Al-Dharrab, K Egashira, W Wisitrasam, K Yamamoto, AI Mira, K Sueishi, X Han, MA Taubman, T Miyamoto, T Kawai OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9 FASEB J., 2018-03-13;0(0):fj201701424R. 2018-03-13 [PMID: 29533736] (Mouse)
• K Sato, A Kato, M Sekai, Y Hamazaki, N Minato Physiologic Thymic Involution Underlies Age-Dependent Accumulation of Senescence-Associated CD4(+) T Cells J. Immunol., 2017-05-24;0(0):. 2017-05-24 [PMID: 28539430] (Mouse)
• MM Johnson, R Mendoza, AJ Raghavendr, R Podila, JM Brown Contribution of engineered nanomaterials physicochemical properties to mast cell degranulation Sci Rep, 2017-03-06;7(0):43570. 2017-03-06 [PMID: 28262689] (Mouse)
• Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption PLoS ONE, 2016-08-11;11(8):e0160622. 2016-08-11 [PMID: 27513935] (Mouse)
• C Trierweiler, B Hockenjos, K Zatloukal, R Thimme, H E Blum, E F Wagner et al. The transcription factor c-JUN/AP-1 promotes HBV-related liver tumorigenesis in mice Cell Death & Differentiation 2016-04-01 [PMID: 26470729] (Transgenic Mouse)
• Bouet G, Bouleftour W, Juignet L, Linossier M, Thomas M, Vanden-Bossche A, Aubin J, Vico L, Marchat D, Malaval L The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent. PLoS ONE, 2015-02-24;10(2):e0117402. 2015-02-24 [PMID: 25710686] (Mouse)
• Bouleftour W, Boudiffa M, Wade-Gueye N, Bouet G, Cardelli M, Laroche N, Vanden-Bossche A, Thomas M, Bonnelye E, Aubin J, Vico L, Lafage-Proust M, Malaval L Skeletal development of mice lacking bone sialoprotein (BSP)--impairment of long bone growth and progressive establishment of high trabecular bone mass. PLoS ONE, 2014-05-09;9(5):e95144. 2014-05-09 [PMID: 24816232] (Mouse)
• Lau , Wei Ling, Leaf , Elizabet, Hu , Ming Cha, Takeno , Marc M, Kuro-o , Makoto, Moe , Orson W, Giachelli , Cecilia Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet. Kidney Int, 2012-08-29;82(12):1261-70. 2012-08-29 [PMID: 22932118] (Mouse)
• Pini M, Rhodes DH, Castellanos KJ Rosiglitazone improves survival and hastens recovery from pancreatic inflammation in obese mice. PLoS ONE, 2012-07-16;7(7):e40944. 2012-07-16 [PMID: 22815875] (Mouse)
• El-Abbadi MM, Pai AS, Leaf EM, Yang HY, Bartley BA, Quan KK, Ingalls CM, Liao HW, Giachelli CM Phosphate feeding induces arterial medial calcification in uremic mice: role of serum phosphorus, fibroblast growth factor-23, and osteopontin. Kidney Int., 2009-03-25;75(12):1297-307. 2009-03-25 [PMID: 19322138] (Mouse)

FAQs for Osteopontin/OPN (DY441). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Spp1