Mouse IL-17E DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-17E DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il25

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-17E DuoSet ELISA, 15 Plate

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Publications for IL-17E/IL-25 (DY1399)(27)

Publications using DY1399

• Lopes, CA;Leal-Silva, T;Vieira-Santos, F;Nascimento Souza, JL;Oliveira, CCA;Oliveira, FMS;Kraemer, L;Magalhaes, L;Bara-Garcia, P;Kang, B;Zamboni, D;Russo, RC;Fujiwara, RT;Nutman, TB;Gazzinelli-Guimaraes, P;Bueno, LL; NOD1 signaling regulates early tissue inflammation during helminth infection Mucosal immunology 2024-12-09 [PMID: 39662675] (Mouse)
• Montasser, A;Dakrory, AE;Ibrahim, MIM;El Zayyat, E;Tallima, H;El Ridi, R; Differential murine responses to Schistosoma mansoni eggs in the liver and small intestine lead to downmodulation of hepatic but not intestinal periovular granulomas Infection and immunity 2024-11-19 [PMID: 39560403] (Mouse)
• Kellogg, TD;Ceglia, S;Mortzfeld, BM;Zeamer, AL;Foley, SE;Ward, DV;Bhattarai, SK;McCormick, BA;Reboldi, A;Bucci, V; Microbiota encoded fatty-acid metabolism expands tuft cells to protect tissues homeostasis during Clostridioides difficile infection in the large intestine bioRxiv : the preprint server for biology 2024-01-31 [PMID: 38352546] (Mouse)
• H Tallima, R El Ridi Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice PloS Neglected Tropical Diseases, 2023-03-15;17(3):e0011164. 2023-03-15 [PMID: 36920999] (Mouse)
• RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu Crosstalk between ILC2s and Th2 cells varies among mouse models Cell Reports, 2023-02-02;42(2):112073. 2023-02-02 [PMID: 36735533] (Mouse)
• J Won, A Jo, CH Gil, S Kim, H Shin, H Jik Kim Inhaled delivery of recombinant interferon-lambda restores allergic inflammation after development of asthma by controlling Th2- and Th17-cell-mediated immune responses International immunopharmacology, 2022-08-27;112(0):109180. 2022-08-27 [PMID: 36030690] (Mouse)
• CR Morris, A Habibovic, CM Dustin, C Schiffers, MC Lin, JL Ather, YMW Janssen-He, ME Poynter, O Utermohlen, M Krönke, A van der Vl Macrophage-intrinsic DUOX1 contributes to type 2 inflammation and mucus metaplasia during allergic airway disease Mucosal Immunology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35654836] (Mouse)
• Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature Cell Biology, 2021-10-06;23(10):1060-1072. 2021-10-06 [PMID: 34616019] (Mouse)
• HJ Jung, YK Ko, WS Shim, HJ Kim, DY Kim, CS Rhee, MK Park, DH Han Diesel exhaust particles increase nasal symptoms and IL-17A in house dust mite-induced allergic mice Scientific Reports, 2021-08-11;11(1):16300. 2021-08-11 [PMID: 34381060] (Mouse)
• CY Chang, J Wang, Y Zhao, J Liu, X Yang, X Yue, H Wang, F Zhou, JM Inclan-Ric, JJ Ponessa, P Xie, L Zhang, MC Siracusa, Z Feng, W Hu Tumor suppressor p53 regulates intestinal type 2 immunity Nature Communications, 2021-06-07;12(1):3371. 2021-06-07 [PMID: 34099671] (Mouse)
• JPK Mukendi, R Nakamura, S Uematsu, S Hamano Interleukin (IL)-33 is dispensable for Schistosoma mansoni worm maturation and the maintenance of egg-induced pathology in intestines of infected mice Parasites & vectors, 2021-01-22;14(1):70. 2021-01-22 [PMID: 33482904] (Mouse)
• CT Wu, YT Lee, MS Ku, KH Lue Role of biomarkers and effect of FIP-fve in acute and chronic animal asthma models J Microbiol Immunol Infect, 2020-07-28;0(0):. 2020-07-28 [PMID: 32778497] (Mouse)
• K Okunishi, H Wang, M Suzukawa, R Ishizaki, E Kobayashi, M Kihara, T Abe, JI Miyazaki, M Horie, A Saito, H Saito, S Nakae, T Izumi Exophilin-5 regulates allergic airway inflammation by controlling IL-33-mediated Th2 responses J. Clin. Invest., 2020-07-01;0(0):. 2020-07-01 [PMID: 32240120] (Mouse)
• EM Nakada, NR Bhakta, BR Korwin-Mih, A Kumar, N Chamberlai, SR Bruno, DG Chapman, SM Hoffman, N Daphtary, M Aliyeva, CG Irvin, AE Dixon, PG Woodruff, S Amin, ME Poynter, DH Desai, V Anathy Conjugated bile acids attenuate allergen-induced airway inflammation and hyperresposiveness by inhibiting UPR transducers JCI Insight, 2019-05-02;4(9):. 2019-05-02 [PMID: 31045581] (Mouse)
• MF Serra, AC Cotias, CRR Pão, JB Daleprane, PB Jurgilas, GC Couto, EA Anjos-Valo, RSB Cordeiro, VF Carvalho, PMR Silva, MA Martins Repeated Allergen Exposure in A/J Mice Causes Steroid-Insensitive Asthma via a Defect in Glucocorticoid Receptor Bioavailability J. Immunol., 2018-06-18;0(0):. 2018-06-18 [PMID: 29914889] (Mouse)
• AR Khosravi, H Shokri, S Hassan Al-, F Ghafarifar Evaluation of murine lung epithelial cells (TC-1 JHU-1) line to develop Th2-promoting cytokines IL-25/IL-33/TSLP and genes Tlr2/Tlr4 in response to Aspergillus fumigatus J Mycol Med, 2018-03-07;0(0):. 2018-03-07 [PMID: 29525270] (Mouse)
• N Gour, S Lajoie, U Smole, M White, D Hu, P Goddard, S Huntsman, C Eng, A Mak, S Oh, JH Kim, A Sharma, S Plante, IH Salem, Y Resch, X Xiao, N Yao, A Singh, S Vrtala, J Chakir, EG Burchard, AP Lane, M Wills-Karp Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases Sci Immunol, 2018-02-23;3(20):. 2018-02-23 [PMID: 29475849] (Mouse)
• K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis PLoS Pathog., 2017-08-17;13(8):e1006513. 2017-08-17 [PMID: 28817707] (Mouse)
• MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection J. Immunol., 2017-06-02;0(0):. 2017-06-02 [PMID: 28576981] (Mouse)
• L Yi, D Cheng, K Zhang, X Huo, Y Mo, H Shi, H Di, Y Zou, H Zhang, J Zhao, Y Xu, DJ Erle, G Zhen Intelectin contributes to allergen-induced IL-25, IL-33, and TSLP expression and type 2 response in asthma and atopic dermatitis Mucosal Immunol, 2017-02-22;0(0):. 2017-02-22 [PMID: 28224996] (Mouse)
• Hiroki Tashiro Interleukin-33 from Monocytes Recruited to the Lung Contributes to House Dust Mite-Induced Airway Inflammation in a Mouse Model PLoS ONE, 2016-06-16;11(6):e0157571. 2016-06-16 [PMID: 27310495] (Mouse)
• JL Reyes, MR Fernando, F Lopes, G Leung, NL Mancini, CE Matisz, A Wang, DM McKay IL-22 Restrains Tapeworm-Mediated Protection against Experimental Colitis via Regulation of IL-25 Expression PLoS Pathog, 2016-04-07;12(4):e1005481. 2016-04-07 [PMID: 27055194] (Mouse)
• Sivapalan , Nirooya, Wattie , Jennifer, Inman , Mark D, Sehmi , Roma Lung-homing of endothelial progenitor cells and airway vascularization is only partially dependant on eosinophils in a house dust mite-exposed mouse model of allergic asthma. PLoS ONE, 2014-10-03;9(10):e109991. 2014-10-03 [PMID: 25279605] (Mouse)
• Wilson , Rhonda H, Maruoka , Shuichir, Whitehead , Gregory, Foley , Julie F, Flake , Gordon P, Sever , Michelle, Zeldin , Darryl C, Kraft , Monica, Garantziotis , Stavros, Nakano , Hideki, Cook , Donald N The Toll-like receptor 5 ligand flagellin promotes asthma by priming allergic responses to indoor allergens. Nat Med, 2012-10-14;18(11):1705-10. 2012-10-14 [PMID: 23064463] (Mouse)
• Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Korner H, Cua D, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide D, Matsumoto K, Nakae S Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, Is crucial for murine asthma. J Immunol, 2012-08-31;189(7):3641-52. 2012-08-31 [PMID: 22942422] (Mouse)
• Interleukin-1alpha controls allergic sensitization to inhaled house dust mite via the epithelial release of GM-CSF and IL-33. J. Exp. Med., 2012-07-16;209(8):1505-17. 2012-07-16 [PMID: 22802353] (Mouse)
• Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J. Immunol., 2011-12-23;188(3):1503-13. 2011-12-23 [PMID: 22198948] (Mouse)

FAQs for IL-17E/IL-25 (DY1399). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il25