Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate Summary

Background
The Quantikine® Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Resul...ts obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.
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Specificity
Natural and recombinant mouse IL-1 beta
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il1b

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
MLB00C in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit, 2 Plate

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

The Interleukin 1 (IL-1) family of proteins consists of IL-1 alpha, IL-1 beta, and the IL-1 receptor antagonist (IL-1ra). IL-1 alpha and IL-1 beta bind to the same cell surface receptors and share biological functions (1). IL-1 is not produced by unstimulated cells of healthy individuals with the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system. However, in response to inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen. IL-1 beta plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. Inappropriate or prolonged production of IL-1 has been implicated in a variety of pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulindependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases (2-5). 
 IL-1 alpha and IL-1 beta are structurally related polypeptides that show approximately 25% homology at the amino acid (aa) level. Both are synthesized as 31 kDa precursors that are subsequently cleaved into mature proteins of approximately 17.5 kDa (6, 7). Cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response (2, 8). Neither IL-1 alpha nor IL-1 beta contains a typical hydrophobic signal peptide (9-11), but evidence suggests that these factors can be secreted by non-classical pathways (12, 13). A portion of unprocessed IL-1 alpha can be presented on the cell membrane and may retain biological activity (14). The precursor form of IL-1 beta, unlike the IL-1 alpha precursor, shows little or no biological activity in comparison to the processed form (13, 15). Both unprocessed and mature forms of IL-1 beta are exported from the cell. 
 IL-1 alpha and IL-1 beta exert their effects through immunoglobulin superfamily receptors that additionally bind IL-1ra. The 80 kDa transmembrane type I receptor (IL-1 RI) is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes (16, 17). The 68 kDa transmembrane type II receptor (IL-1 RII) is expressed on B cells, neutrophils, and bone marrow cells (18). The two IL-1 receptor types show approximately 28% homology in their extracellular domains but differ significantly in that the type II receptor has a cytoplasmic domain of only 29 aa, whereas the type I receptor has a 213 aa cytoplasmic domain. IL-1 RII does not appear to signal in response to IL-1 and may function as a decoy receptor that attenuates IL-1 function (19). The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction (20). IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 (21, 22). Soluble forms of both IL-1 RI and IL-1 RII have been detected in human plasma, synovial fluids, and the conditioned media of several human cell lines (23, 24). In addition, IL-1 binding proteins that resemble soluble IL-1 RII are encoded by vaccinia and cowpox viruses (25).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1 beta/IL-1F2 (MLB00C)(284)

We have publications tested in 3 confirmed species: Mouse, Rat, Guinea Pig.

We have publications tested in 2 applications: ELISA Capture, data reported.


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(3)
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(1)
Guinea Pig
(1)
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Showing Publications 1 - 10 of 284. Show All 284 Publications.
Publications using MLB00C Applications Species
X Wu, S Pan, W Luo, Z Shen, X Meng, M Xiao, B Tan, K Nie, T Tong, X Wang Roseburia�intestinalis?derived flagellin ameliorates colitis by targeting miR?223?3p?mediated activation of NLRP3 inflammasome and pyroptosis Mol Med Rep, 2020;0(0):. 2020 [PMID: 32700754] (Mouse) Mouse
HF Zhang, HB Zhang, XP Wu, YL Guo, WD Cheng, F Qian Fisetin alleviates sepsis-induced multiple organ dysfunction in mice via inhibiting p38 MAPK/MK2 signaling Acta Pharmacol. Sin., 2020;0(0):. 2020 [PMID: 32661350] (Mouse) Mouse
WC Chen, NJ Chen, HP Chen, WK Yu, VY Su, H Chen, HH Wu, KY Yang Nintedanib Reduces Neutrophil Chemotaxis via Activating GRK2 in Bleomycin-Induced Pulmonary Fibrosis Int J Mol Sci, 2020;21(13):. 2020 [PMID: 32630825] (Mouse) Mouse
ST Keating, L Groh, CDCC van der He, H Rodriguez, JC Dos Santos, S Fanucchi, J Okabe, H Kaipananic, JH van Puffel, L Helder, MP Noz, V Matzaraki, Y Li, LCJ de Bree, VACM Koeken, SJCFM Moorlag, VP Mourits, J Domínguez-, M Oosting, EP Bulthuis, WJH Koopman, M Mhlanga, A El-Osta, LAB Joosten, MG Netea, NP Riksen The Set7 Lysine Methyltransferase Regulates Plasticity in Oxidative Phosphorylation Necessary for Trained Immunity Induced by &beta-Glucan Cell Rep, 2020;31(3):107548. 2020 [PMID: 32320649] (Mouse) Mouse
W Shi, G Xu, X Zhan, Y Gao, Z Wang, S Fu, N Qin, X Hou, Y Ai, C Wang, T He, H Liu, Y Chen, Y Liu, J Wang, M Niu, Y Guo, X Xiao, Z Bai Carnosol inhibits inflammasome activation by directly targeting HSP90 to treat inflammasome-mediated diseases Cell Death Dis, 2020;11(4):252. 2020 [PMID: 32312957] (Mouse) Mouse
N Wada, H Yamada, S Motoyama, M Saburi, T Sugimoto, H Kubota, D Miyawaki, N Wakana, D Kami, T Ogata, S Matoba Maternal high-fat diet exaggerates diet-induced insulin resistance in adult offspring by enhancing inflammasome activation through noncanonical pathway of caspase-11 Mol Metab, 2020;37(0):100988. 2020 [PMID: 32272237] (Mouse) Mouse
LR Ling, DH Zheng, ZY Zhang, WH Xie, YH Huang, ZX Chen, XZ Wang, D Li Effect of HBx on inflammation and mitochondrial oxidative stress in mouse hepatocytes Oncol Lett, 2020;19(4):2861-2869. 2020 [PMID: 32218840]
A Famà, A Midiri, G Mancuso, C Biondo, G Lentini, R Galbo, MM Giardina, GV De Gaetano, L Romeo, G Teti, C Beninati Nucleic Acid-Sensing Toll-Like Receptors Play a Dominant Role in Innate Immune Recognition of Pneumococci MBio, 2020;11(2):. 2020 [PMID: 32209688] (Mouse) Mouse
F Lin, Y Zheng, L Pan, Z Zuo Attenuation of noisy environment-induced neuroinflammation and dysfunction of learning and memory by minocycline during perioperative period in mice Brain Res. Bull., 2020;159(0):16-24. 2020 [PMID: 32208177] (Mouse) Mouse
C Ge, L Hu, D Lou, Q Li, J Feng, Y Wu, J Tan, M Xu Nrf2 deficiency aggravates PM2.5-induced cardiomyopathy by enhancing oxidative stress, fibrosis and inflammation via RIPK3-regulated mitochondrial disorder Aging (Albany NY), 2020;12(6):4836-4865. 2020 [PMID: 32182211] (Mouse) Mouse
Show All 284 Publications.

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Product General Protocols

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FAQs for IL-1 beta/IL-1F2 (MLB00C). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MLB00C
HRP MLB00C
HRP MLB00C

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Bioinformatics

Gene Symbol Il1b