Mouse Coagulation Factor III/TF DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse Coagulation Factor III/TF DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: F3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Coagulation Factor III/TF DuoSet ELISA, 5 Plate

• CD142 antigen
• CD142
• coagulation factor III (thromboplastin, tissue factor)
• Coagulation Factor III
• F3
• FLJ17960
• TF
• TFA
• Thromboplastin
• Tissue Factor

Background

Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.

Publications for Coagulation Factor III/Tissue Factor (DY3178-05)(3)

Publications using DY3178-05

• Puspitasari, YM;Ministrini, S;Han, J;Karch, C;Prisco, F;Liberale, L;Bengs, S;Akhmedov, A;Montecucco, F;Beer, JH;Lüscher, TF;Bongiovanni, D;Camici, GG; Hutchinson-Gilford progeria syndrome mice display accelerated arterial thrombus formation and increased platelet reactivity Thrombosis research 2024-07-18 [PMID: 39032390] (Transgenic Mouse)
• SO Vasudevan, AJ Russo, P Kumari, SK Vanaja, VA Rathinam A TLR4-independent critical role for CD14 in intracellular LPS sensing Cell Reports, 2022-05-03;39(5):110755. 2022-05-03 [PMID: 35508125] (Mouse)
• DS Gaul, J Weber, LJ Van Tits, S Sluka, L Pasterk, MF Reiner, N Calatayud, C Lohmann, R Klingenber, J Pahla, D Vdovenko, FC Tanner, GG Camici, U Eriksson, J Auwerx, F Mach, S Windecker, N Rodondi, TF L Uuml Sch, S Winnik, CM Matter Loss of Sirt3 accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activity Cardiovasc. Res., 2018-07-01;0(0):. 2018-07-01 [PMID: 29444200] (Mouse)

FAQs for Coagulation Factor III/Tissue Factor (DY3178-05). (Showing 1 - 2 of 2 FAQs).

1. Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.
   • Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: F3