Mouse CD25/IL-2R alpha DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CD25/IL-2R alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il2ra

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CD25/IL-2R alpha DuoSet ELISA, 15 Plate

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.

Publications for CD25/IL-2R alpha (DY2438)(11)

Publications using DY2438

• Mann, J;Runge, S;Schell, C;Gräwe, K;Thoulass, G;Lao, J;Ammann, S;Grün, S;König, C;Berger, SA;Hild, B;Aichele, P;Rosshart, SP;Ehl, S; The Microbiome Modifies Manifestations of Hemophagocytic Lymphohistiocytosis in Perforin-Deficient Mice European journal of immunology 2024-11-16 [PMID: 39548906] (Transgenic Mouse)
• Yap, J;Yuan, J;Ng, WH;Chen, GB;Sim, YRM;Goh, KC;Teo, J;Lim, TYH;Goay, SM;Teo, JHJ;Lao, Z;Lam, P;Sabapathy, K;Hu, J; BRAF(V600E) mutation together with loss of Trp53 or pTEN drives the origination of hairy cell leukemia from B-lymphocytes Molecular cancer 2023-08-05 [PMID: 37543582] (Mouse)
• Diamond, T;Lau, M;Morrissette, J;Chu, N;Behrens, EM; CXCL9 inhibition does not ameliorate disease in murine models of both primary and secondary hemophagocytic lymphohistiocytosis Scientific reports 2023-07-29 [PMID: 37516815] (Mouse)
• K Wei beta ert, S Ammann, T Kögl, V Dettmer-Mo, C Schell, T Cathomen, S Ehl, P Aichele Adoptive T cell therapy cures mice from active hemophagocytic lymphohistiocytosis (HLH) Embo Molecular Medicine, 2022-10-24;0(0):e16085. 2022-10-24 [PMID: 36278424] (Mouse)
• T Diamond, TN Burn, MA Nishiguchi, D Minichino, J Chase, N Chu, PA Kreiger, EM Behrens Familial hemophagocytic lymphohistiocytosis hepatitis is mediated by IFN-gamma in a predominantly hepatic-intrinsic manner PLoS ONE, 2022-06-07;17(6):e0269553. 2022-06-07 [PMID: 35671274] (Mouse)
• S Kirschke, I Ogunsulire, B Selvakumar, N Schumacher, T Sezin, S Rose-John, A Scheffold, C Garbers, J Lokau The metalloprotease ADAM10 generates soluble interleukin-2 receptor alpha (sIL-2Ralpha/sCD25) in vivo The Journal of Biological Chemistry, 2022-04-07;0(0):101910. 2022-04-07 [PMID: 35398356] (Mouse)
• R Gather, P Aichele, N Goos, J Rohr, H Pircher, T Kögl, R Zeiser, H Hengel, A Schmitt-Gr, C Weaver, S Ehl Trigger-dependent differences determine therapeutic outcome in murine primary hemophagocytic lymphohistiocytosis Eur. J. Immunol., 2020-06-02;0(0):. 2020-06-02 [PMID: 32419134] (Mouse)
• SJ Tavernier, V Athanasopo, P Verloo, G Behrens, J Staal, DJ Bogaert, L Naesens, M De Bruyne, S Van Gassen, E Parthoens, J Ellyard, J Cappello, LX Morris, H Van Gorp, G Van Isterd, Y Saeys, M Lamkanfi, P Schelstrae, J Dehoorne, V Bordon, R Van Coster, BN Lambrecht, B Menten, R Beyaert, CG Vinuesa, V Heissmeyer, M Dullaers, F Haerynck A human immune dysregulation syndrome characterized by severe hyperinflammation with a homozygous nonsense Roquin-1 mutation Nat Commun, 2019-10-21;10(1):4779. 2019-10-21 [PMID: 31636267] (Mouse)
• EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells Nature, 2016-05-05;533(7601):110-4. 2016-05-05 [PMID: 27147029] (Mouse)
• Jessen B, Maul-Pavicic A, Ufheil H, Vraetz T, Enders A, Lehmberg K, Langler A, Gross-Wieltsch U, Bay A, Kaya Z, Bryceson YT, Koscielniak E, Badawy S, Davies G, Hufnagel M, Schmitt-Graeff A, Aichele P, Zur Stadt U, Schwarz K, Ehl S Subtle differences in CTL cytotoxicity determine susceptibility to hemophagocytic lymphohistiocytosis in mice and humans with Chediak-Higashi syndrome. Blood, 2011-08-30;118(17):4620-9. 2011-08-30 [PMID: 21878672] (Mouse)
• Secor ER, Singh A, Guernsey LA Bromelain treatment reduces CD25 expression on activated CD4+ T cells in vitro. Int. Immunopharmacol., 2009-01-20;9(3):340-6. 2009-01-20 [PMID: 19162239] (Mouse)

FAQs for CD25/IL-2R alpha (DY2438). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il2ra