Mouse CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL3/MIP-1 alpha DuoSet ELISA, 15 Plate

• C-C motif chemokine 3
• MIP1-(a)
• AI323804
• CCL3
• chemokine (C-C motif) ligand 3
• G0S19-1
• LD78a
• LD78alpha
• MIP1 alpha
• MIP-1 alpha
• MIP1A
• MIP-1alpha
• MIP1-alpha
• PAT 464.1
• SCYA3
• SIS-beta

Background

MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.

The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.

Publications for CCL3/MIP-1 alpha (DY450)(56)

Publications using DY450

• Watanabe, T;Hayashi, M;Arai, M;Matsushita, S;Handa, H;Kawano, M; Interferon-? production in response to the cytotoxic T lymphocyte epitope within an antigen incorporated in simian virus 40 virus-like particles Heliyon 2025-01-30 [PMID: 39897799] (Mouse)
• Kent, A;Yee Mon, KJ;Hutchins, Z;Putzel, G;Zhigarev, D;Grier, A;Jia, B;Kortlever, RM;Barbet, G;Evan, GI;Blander, JM; A stromal inflammasome Ras safeguard against Myc-driven lymphomagenesis Nature immunology 2025-01-01 [PMID: 39747433] (Mouse, Transgenic Mouse)
• de Oliveira, LD;de Carvalho, LS;Xavier, ACC;de Oliveira, FE;Leão, MVP;Diamantino, MGG;Khoury, RD;Valera, MC;Carvalho, CAT;Abu Hasna, A; In Vitro Evaluation of Sodium Hypochlorite, Chlorhexidine, Propolis, and Calcium Hydroxide Effect on Lipoteichoic-Acid-Induced Proinflammatory Cytokines Production Dentistry journal 2024-09-05 [PMID: 39329852] (Mouse)
• Sun, L;Apweiler, M;Tirkey, A;Klett, D;Normann, C;Dietz, GPH;Lehner, MD;Fiebich, BL; Anti-Neuroinflammatory Effects of Ginkgo biloba Extract EGb 761 in LPS-Activated BV2 Microglial Cells International journal of molecular sciences 2024-07-25 [PMID: 39125680] (Mouse)
• Sun, L;Wilke Saliba, S;Apweiler, M;Akmermer, K;Herlan, C;Grathwol, C;de Oliveira, ACP;Normann, C;Jung, N;Bräse, S;Fiebich, BL; Anti-Neuroinflammatory Effects of a Macrocyclic Peptide-Peptoid Hybrid in Lipopolysaccharide-Stimulated BV2 Microglial Cells International journal of molecular sciences 2024-04-18 [PMID: 38674048] (Mouse)
• Kim, YT;Huh, JW;Choi, YH;Yoon, HK;Nguyen, TT;Chun, E;Jeong, G;Park, S;Ahn, S;Lee, WK;Noh, YW;Lee, KS;Ahn, HS;Lee, C;Lee, SM;Kim, KS;Suh, GJ;Jeon, K;Kim, S;Jin, M; Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis EMBO molecular medicine 2024-01-01 [PMID: 38177528] (Mouse)
• Nyariki, JN;Kimani, NM;Kibet, PS;Kinuthia, GK;Isaac, AO; Coenzyme Q10 exhibits anti-inflammatory and immune-modulatory thereby decelerating the occurrence of experimental cerebral malaria Molecular and biochemical parasitology 2023-06-27 [PMID: 37385350] (Mouse)
• N Zeren, Z Afzal, S Morgan, G Marshall, M Uppiliappa, J Merritt, SJ Coniglio The Chemokine Receptor CCR1 Mediates Microglia Stimulated Glioma Invasion International Journal of Molecular Sciences, 2023-03-07;24(6):. 2023-03-07 [PMID: 36982211] (Mouse)
• TTT Nguyen, YH Choi, WK Lee, Y Ji, E Chun, YH Kim, JE Lee, HS Jung, JH Suh, S Kim, M Jin Tryptophan-dependent and -independent secretions of tryptophanyl- tRNA synthetase mediate innate inflammatory responses Cell Reports, 2022-12-30;42(1):111905. 2022-12-30 [PMID: 36640342] (Mouse)
• P Selvam, CM Cheng, HU Dahms, VK Ponnusamy, YY Sun AhR Mediated Activation of Pro-Inflammatory Response of RAW 264.7 Cells Modulate the Epithelial-Mesenchymal Transition Toxics, 2022-10-27;10(11):. 2022-10-27 [PMID: 36355934] (Mouse)
• Y Fukushima, K Sakamoto, M Matsuda, Y Yoshikai, H Yagita, D Kitamura, M Chihara, N Minato, M Hattori cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T�cells Cell Reports, 2022-09-20;40(12):111373. 2022-09-20 [PMID: 36130493] (Mouse)
• A Dietschman, S Schruefer, S Westermann, F Henkel, K Castiglion, R Willebrand, J Adam, J Ruland, R Lang, DC Sheppard, J Esser-von-, D Radtke, S Krappmann, D Voehringer Phosphatidylinositol 3-Kinase (PI3K) Orchestrates Aspergillus fumigatus-Induced Eosinophil Activation Independently of Canonical Toll-Like Receptor (TLR)/C-Type-Lectin Receptor (CLR) Signaling MBio, 2022-06-13;0(0):e0123922. 2022-06-13 [PMID: 35695427] (Mouse)
• T Arsiwala, AS Vogt, AE Barton, V Manolova, F Funk, B Flühmann, MF Bachmann Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from Serum International Journal of Molecular Sciences, 2022-02-28;23(5):. 2022-02-28 [PMID: 35269805] (Mouse)
• E Zajta, K Csonka, A Tóth, L Tiszlavicz, T Németh, A Orosz, Á Novák, M Csikós, C Vágvölgyi, A Mócsai, A Gácser Signaling through Syk or CARD9 Mediates Species-Specific Anti-Candida Protection in Bone Marrow Chimeric Mice MBio, 2021-08-31;12(4):e0160821. 2021-08-31 [PMID: 34465030] (Mouse, Transgenic Mouse)
• MTP de Oliveir, DS Coutinho, SS Guterres, AR Pohlmann, PMRE Silva, MA Martins, A Bernardi Resveratrol-Loaded Lipid-Core Nanocapsules Modulate Acute Lung Inflammation and Oxidative Imbalance Induced by LPS in Mice Pharmaceutics, 2021-05-10;13(5):. 2021-05-10 [PMID: 34068619] (Mouse)
• S Ribes, L Zacke, S Nessler, N Saiepour, E Avendaño-G, M Ballüer, UK Hanisch, R Nau Oligodeoxynucleotides containing unmethylated cytosine-guanine motifs are effective immunostimulants against pneumococcal meningitis in the immunocompetent and neutropenic host Journal of Neuroinflammation, 2021-02-02;18(1):39. 2021-02-02 [PMID: 33531028] (Mouse)
• C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting Nat. Immunol., 2020-08-31;0(0):. 2020-08-31 [PMID: 32868928] (Mouse)
• MB Reis, J Elias-Oliv, MR Pastore, SG Ramos, LG Gardinassi, LH Faccioli Interleukin-1 Receptor-Induced Nitric Oxide Production in the Pancreas Controls Hyperglycemia Caused by Scorpion Envenomation Toxins (Basel), 2020-03-05;12(3):. 2020-03-05 [PMID: 32150895] (Mouse)
• S Ribes, C Arcilla, M Ott, S Schütze, UK Hanisch, S Nessler, R Nau Pre-treatment with the viral Toll-like receptor 3 agonist poly(I:C) modulates innate immunity and protects neutropenic mice infected intracerebrally with Escherichia coli J Neuroinflammation, 2020-01-17;17(1):24. 2020-01-17 [PMID: 31952519] (Mouse)
• E Morello, T Pérez-Bere, C Boisseau, T Baranek, A Guillon, D Bréa, P Lanotte, X Carpena, N Pietrancos, V Hervé, R Ramphal, N Cenac, M Si-Tahar Pseudomonas aeruginosa Lipoxygenase LoxA Contributes to Lung Infection by Altering the Host Immune Lipid Signaling Front Microbiol, 2019-08-14;10(0):1826. 2019-08-14 [PMID: 31474948] (Mouse)
• VN Camargos, G Foureaux, DC Medeiros, VT da Silveir, CM Queiroz-Ju, ALB Matosinhos, AFA Figueiredo, CDF Sousa, TP Moreira, VF Queiroz, ACF Dias, KTO Santana, I Passos, ALCV Real, LC Silva, FAG Mourão, NT Wnuk, MAP Oliveira, S Macari, T Silva, GP Garlet, JA Jackman, FM Soriani, MFD Moraes, EMAM Mendes, FM Ribeiro, GMJ Costa, AL Teixeira, NJ Cho, ACP Oliveira, MM Teixeira, VV Costa, DG Souza In-depth characterization of congenital Zika syndrome in immunocompetent mice: Antibody-dependent enhancement and an antiviral peptide therapy EBioMedicine, 2019-05-23;0(0):. 2019-05-23 [PMID: 31130472] (Mouse)
• E Martin, M Amar, C Dalle, I Youssef, C Boucher, C Le Duigou, M Brückner, A Prigent, V Sazdovitch, A Halle, JM Kanellopou, B Fontaine, B Delatour, C Delarasse New role of P2X7 receptor in an Alzheimer's disease mouse model Mol. Psychiatry, 2018-06-22;0(0):. 2018-06-22 [PMID: 29934546] (Mouse)
• TM Finlay, AL Palmer, SS Ousman Murine neutrophils treated with alphaB-crystallin reduce IL-12p40 production by dendritic cells Immunology, 2018-04-17;0(0):. 2018-04-17 [PMID: 29532462] (Mouse)
• M Lynch, TA Walsh, I Marszalows, AE Webb, M MacAogain, TR Rogers, H Windle, D Kelleher, MJ O'Connell, CE Loscher Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response BMC Evol. Biol, 2017-03-23;17(1):90. 2017-03-23 [PMID: 28335725] (Mouse)
• CM Henry, SJ Martin Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation Mol. Cell, 2017-02-16;65(4):715-729.e5. 2017-02-16 [PMID: 28212752] (Mouse)
• SB Fleming, C McCaughan, Z Lateef, A Dunn, LM Wise, NC Real, AA Mercer Deletion of the Chemokine Binding Protein Gene from the Parapoxvirus Orf Virus Reduces Virulence and Pathogenesis in Sheep Front Microbiol, 2017-01-24;8(0):46. 2017-01-24 [PMID: 28174562] (Mouse)
• The Carbohydrate Lectin Receptor, Dectin-1, Mediates Immune Response to Exserohilum rostratum Infect. Immun, 2017-02-23;0(0):. 2017-02-23 [PMID: 28031265] (Mouse)
• Tatiana Paula Teixeira Ferreira, Lívia Lacerda Mariano, Roberta Ghilosso-Bortolini, Ana Carolina Santos de de Arantes, Andrey Junior Fernandes, Michelle Berni et al. Potential of PEGylated Toll-Like Receptor 7 Ligands for Controlling Inflammation and Functional Changes in Mouse Models of Asthma and Silicosis Frontiers in Immunology 2016-03-11 [PMID: 27014274] (Mouse)
• Carlucci F, Ishaque A, Ling G, Szajna M, Sandison A, Donatien P, Cook H, Botto M C1q Modulates the Response to TLR7 Stimulation by Pristane-Primed Macrophages: Implications for Pristane-Induced Lupus. J Immunol, 2016-01-15;196(4):1488-94. 2016-01-15 [PMID: 26773156] (Mouse)
• Yoshida K, Maekawa T, Zhu Y, Renard-Guillet C, Chatton B, Inoue K, Uchiyama T, Ishibashi K, Yamada T, Ohno N, Shirahige K, Okada-Hatakeyama M, Ishii S The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory. Nat Immunol, 2015-08-31;16(10):1034-43. 2015-08-31 [PMID: 26322480] (Mouse)
• Todd D, Gulledge T, Britton E, Oberhofer M, Leyte-Lugo M, Moody A, Shymanovich T, Grubbs L, Juzumaite M, Graf T, Oberlies N, Faeth S, Laster S, Cech N Ethanolic Echinacea purpurea Extracts Contain a Mixture of Cytokine-Suppressive and Cytokine-Inducing Compounds, Including Some That Originate from Endophytic Bacteria. PLoS ONE, 2015-05-01;10(5):e0124276. 2015-05-01 [PMID: 25933416] (Mouse)
• Bernard N, Finlay C, Tannahill G, Cassidy J, O'Neill L, Mills K A critical role for the TLR signaling adapter Mal in alveolar macrophage-mediated protection against Bordetella pertussis. Mucosal Immunol, 2014-12-17;8(5):982-92. 2014-12-17 [PMID: 25515629] (Mouse)
• Goncalves-de-Albuquerque C, Burth P, Silva A, de Moraes I, Oliveira F, Santelli R, Freire A, de Lima G, da Silva E, da Silva C, Morandi V, Bozza P, Younes-Ibrahim M, de Castro Faria Neto H, de Castro Faria M Murine lung injury caused by Leptospira interrogans glycolipoprotein, a specific Na/K-ATPase inhibitor. Respir Res, 2014-08-14;15(0):93. 2014-08-14 [PMID: 25265888] (Mouse)
• Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection. J Neuroinflammation, 2014-01-23;11(0):14. 2014-01-23 [PMID: 24456653] (Mouse)
• Menezes-Garcia Z, Oliveira M, Lima R, Soriani F, Cisalpino D, Botion L, Teixeira M, Souza D, Ferreira A Lack of platelet-activating factor receptor protects mice against diet-induced adipose inflammation and insulin-resistance despite fat pad expansion. Obesity (Silver Spring), 2013-12-14;22(3):663-72. 2013-12-14 [PMID: 24339378] (Mouse)
• Ryser S, Schuppli M, Gauthier B, Hernandez D, Roye O, Hohl D, German B, Holzwarth J, Moodycliffe A UVB-induced skin inflammation and cutaneous tissue injury is dependent on the MHC class I-like protein, CD1d. J Invest Dermatol, 2013-07-18;134(1):192-202. 2013-07-18 [PMID: 23867896] (Mouse)
• Fevre C, Almeida A, Taront S, Pedron T, Huerre M, Prevost M, Kieusseian A, Cumano A, Brisse S, Sansonetti P, Tournebize R A novel murine model of rhinoscleroma identifies Mikulicz cells, the disease signature, as IL-10 dependent derivatives of inflammatory monocytes. EMBO Mol Med, 2013-04-01;5(4):516-30. 2013-04-01 [PMID: 23554169] (Mouse)
• Wu Z, MacNeil A, Berman J, Lin T Syntaxin binding protein 1 is not required for allergic inflammation via IgE-mediated mast cell activation. PLoS ONE, 2013-03-06;8(3):e58560. 2013-03-06 [PMID: 23484036] (Mouse)
• Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection. Infect. Immun., 2011-10-24;80(1):100-9. 2011-10-24 [PMID: 22025515] (Mouse)
• van Neerven S, Regen T, Wolf D, Nemes A, Johann S, Beyer C, Hanisch UK, Mey J Inflammatory chemokine release of astrocytes in vitro is reduced by all-trans retinoic acid. J. Neurochem., 2010-06-16;114(5):1511-26. 2010-06-16 [PMID: 20557428] (Mouse)
• Graham BS, Kines RC, Corbett KS, Nicewonger J, Roberts JN, Schiller JT, Buck CB Mucosal delivery of human papillomavirus pseudovirus-encapsidated plasmids improves the potency of DNA vaccination. Mucosal Immunol, 2010-06-16;3(5):475-86. 2010-06-16 [PMID: 20555315] (Mouse)
• Wan H, Coppens JM, van Helden-Meeuwsen CG, Leenen PJ, Van Rooijen N, Khan NA, Kiekens RC, Benner R, Versnel MA Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function. J. Leukoc. Biol., 2009-05-04;86(2):361-70. 2009-05-04 [PMID: 19414540] (Mouse)
• Wu Y, Li YY, Matsushima K, Baba T, Mukaida N CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J. Immunol., 2008-11-01;181(9):6384-93. 2008-11-01 [PMID: 18941229] (Mouse)
• Tramonti D, Rhodes K, Martin N, Dalton JE, Andrew E, Carding SR gammadeltaT cell-mediated regulation of chemokine producing macrophages during Listeria monocytogenes infection-induced inflammation. J. Pathol., 2008-10-01;216(2):262-70. 2008-10-01 [PMID: 18767021] (Mouse)
• Tiwari N, Wang CC, Brochetta C, Ke G, Vita F, Qi Z, Rivera J, Soranzo MR, Zabucchi G, Hong W, Blank U VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways. Blood, 2008-01-18;111(7):3665-74. 2008-01-18 [PMID: 18203950] (Mouse)
• Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection. J. Immunol., 2008-01-15;180(2):1080-7. 2008-01-15 [PMID: 18178848] (Mouse)
• Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes. J. Immunol., 2007-12-15;179(12):8381-91. 2007-12-15 [PMID: 18056384] (Human)
• Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice. J. Immunol., 2007-06-15;178(12):8090-6. 2007-06-15 [PMID: 17548647] (Mouse)
• Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity. Immunity, 2007-04-12;26(4):445-59. 2007-04-12 [PMID: 17433730] (Mouse)
• Tramonti D, Andrew EM, Rhodes K, Newton DJ, Carding SR Evidence for the opposing roles of different gamma delta T cell subsets in macrophage homeostasis. Eur. J. Immunol., 2006-07-01;36(7):1729-38. 2006-07-01 [PMID: 16783854] (Mouse)
• Vizzardelli C, Pavelka N, Luchini A, Zanoni I, Bendickson L, Pelizzola M, Beretta O, Foti M, Granucci F, Nilsen-Hamilton M, Ricciardi-Castagnoli P Effects of dexamethazone on LPS-induced activationand migration of mouse dendritic cells revealed by a genome-wide transcriptional analysis. Eur. J. Immunol., 2006-06-01;36(6):1504-15. 2006-06-01 [PMID: 16708398] (Mouse)
• Tengvall S, Lundqvist A, Eisenberg RJ, Cohen GH, Harandi AM Mucosal administration of CpG oligodeoxynucleotide elicits strong CC and CXC chemokine responses in the vagina and serves as a potent Th1-tilting adjuvant for recombinant gD2 protein vaccination against genital herpes. J. Virol., 2006-06-01;80(11):5283-91. 2006-06-01 [PMID: 16699008] (Mouse)
• Rodriguez N, Wantia N, Fend F, Durr S, Wagner H, Miethke T Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae. Eur. J. Immunol., 2006-05-01;36(5):1145-55. 2006-05-01 [PMID: 16609927] (Mouse)
• Carpenter KJ, Hogaboam CM Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis. Infect. Immun., 2005-11-01;73(11):7198-207. 2005-11-01 [PMID: 16239514] (Mouse)
• Okamatsu Y, Battaglino R, Spate U, Stashenko P MIP-1 gamma promotes receptor-activator-of-NF-kappa-B-ligand-induced osteoclast formation and survival. J. Immunol., 2004-08-01;173(3):2084-90. 2004-08-01 [PMID: 15265944] (Mouse)
• Punturieri A, Alviani RS, Polak T, Copper P, Sonstein J, Curtis JL Specific engagement of TLR4 or TLR3 does not lead to IFN-beta-mediated innate signal amplification and STAT1 phosphorylation in resident murine alveolar macrophages. J. Immunol., 2004-07-15;173(2):1033-42. 2004-07-15 [PMID: 15240691] (Mouse)

FAQs for CCL3/MIP-1 alpha (DY450). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL3