Mouse CCL21/6Ckine DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse CCL21/6Ckine DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ccl21a

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse CCL21/6Ckine DuoSet ELISA, 15 Plate

• 6Ckine
• 6CkineSmall-inducible cytokine A21
• Beta-chemokine exodus-2
• CCL21
• chemokine (C-C motif) ligand 21
• CKb9
• exodus-2
• member 21
• SCYA21
• SCYA21MGC34555
• secondary lymphoid tissue chemokine
• SLC
• SLCSecondary lymphoid-tissue chemokine
• TCA-4

Background

CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a homeostatic chemokine that signals through CCR7 or CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC) where it promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. Its upregulation during chronic inflammation or tissue damage promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients.

Publications for CCL21/6Ckine (DY457)(23)

Publications using DY457

• Baek, BS;Park, H;Choi, JW;Lee, EY;Seong, SY; HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment Pharmaceutics 2025-01-06 [PMID: 39861713] (Mouse)
• Morgaenko, K;Arneja, A;Ball, AG;Putelo, AM;Munson, JM;Rutkowski, MR;Pompano, RR; Ex vivo model of breast cancer cell invasion in live lymph node tissue bioRxiv : the preprint server for biology 2024-07-22 [PMID: 39091774] (Mouse)
• Hopkin, SJ;Pezhman, L;Begum, J;Kavanagh, D;McGettrick, HM;Iqbal, AJ;Chimen, M; Aging modulates homeostatic leukocyte trafficking to the peritoneal cavity in a sex-specific manner Journal of leukocyte biology 2023-06-13 [PMID: 37309034] (Mouse)
• JD Cherry, ZH Baucom, KG Eppich, D Kirsch, ER Dixon, Y Tripodis, KF Bieniek, K Farrell, K Whitney, M Uretsky, JF Crary, D Dickson, AC McKee Neuroimmune proteins can differentiate between tauopathies Journal of Neuroinflammation, 2022-11-19;19(1):278. 2022-11-19 [PMID: 36403052] (Human)
• HP Hsu, YT Chen, YY Chen, CY Lin, PY Chen, SY Liao, CCY Lim, Y Yamaguchi, CL Hsu, IL Dzhagalov Heparan sulfate is essential for thymus growth The Journal of Biological Chemistry, 2021-02-15;0(0):100419. 2021-02-15 [PMID: 33600795] (Mouse)
• Y Huang, Y Chen, S Zhou, L Chen, J Wang, Y Pei, M Xu, J Feng, T Jiang, K Liang, S Liu, Q Song, G Jiang, X Gu, Q Zhang, X Gao, J Chen Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors Nat Commun, 2020-01-30;11(1):622. 2020-01-30 [PMID: 32001695] (Mouse)
• L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19 Oncol Lett, 2018-08-09;16(4):5013-5019. 2018-08-09 [PMID: 30250567] (Mouse)
• W Piao, Y Xiong, K Famulski, CC Brinkman, L Li, N Toney, C Wagner, V Saxena, T Simon, JS Bromberg Regulation of T cell afferent lymphatic migration by targeting LT?R-mediated non-classical NF?B signaling Nat Commun, 2018-08-01;9(1):3020. 2018-08-01 [PMID: 30069025] (Mouse)
• V Besnard, R Dagher, T Madjer, A Joannes, M Jaillet, M Kolb, P Bonniaud, LA Murray, MA Sleeman, B Crestani Identification of periplakin as a major regulator of lung injury and repair in mice JCI Insight, 2018-03-08;3(5):. 2018-03-08 [PMID: 29515024] (Mouse)
• W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection Cytokine, 2018-02-01;0(0):. 2018-02-01 [PMID: 29396045] (Mouse)
• JL Gregory, A Walter, YO Alexandre, JL Hor, R Liu, JZ Ma, S Devi, N Tokuda, Y Owada, LK Mackay, GK Smyth, WR Heath, SN Mueller Infection Programs Sustained Lymphoid Stromal Cell Responses and Shapes Lymph Node Remodeling upon Secondary Challenge Cell Rep, 2017-01-10;18(2):406-418. 2017-01-10 [PMID: 28076785] (Mouse)
• Richner J, Gmyrek G, Govero J, Tu Y, van der Windt G, Metcalf T, Haddad E, Textor J, Miller M, Diamond M Age-Dependent Cell Trafficking Defects in Draining Lymph Nodes Impair Adaptive Immunity and Control of West Nile Virus Infection. PLoS Pathog, 2015-07-23;11(7):e1005027. 2015-07-23 [PMID: 26204259] (Mouse)
• Alissafi T, Hatzioannou A, Ioannou M, Sparwasser T, Grun J, Grutzkau A, Verginis P De novo-induced self-antigen-specific Foxp3+ regulatory T cells impair the accumulation of inflammatory dendritic cells in draining lymph nodes. J Immunol, 2015-05-06;194(12):5812-24. 2015-05-06 [PMID: 25948818] (Mouse)
• Lucas B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, Jenkinson W, Anderson G, Rot A CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice. Eur J Immunol, 2015-01-16;45(2):574-83. 2015-01-16 [PMID: 25521433] (Mouse)
• White C, Villarino N, Sloan S, Ganusov V, Schmidt N Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol, 2014-12-10;194(2):697-708. 2014-12-10 [PMID: 25505280] (Mouse)
• Neill , Daniel R, Saint , Gemma L, Bricio-Moreno , Laura, Fothergill , Joanne L, Southern , Kevin W, Winstanley , Craig, Christmas , Stephen, Slupsky , Joseph R, McNamara , Paul S, Kadioglu , Aras, Flanagan , Brian F The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa. PLoS ONE, 2014-05-21;9(5):e95892. 2014-05-21 [PMID: 24847941] (Mouse)
• Paradis M, Mindt B, Duerr C, Rojas O, Ng D, Boulianne B, McCarthy D, Yu M, Summers deLuca L, Ward L, Waldron J, Philpott D, Gommerman J, Fritz J A TNF-alpha-CCL20-CCR6 axis regulates Nod1-induced B cell responses. J Immunol, 2014-02-17;192(6):2787-99. 2014-02-17 [PMID: 24534531] (Mouse)
• Maina , Virginia, Marrella , Veronica, Mantero , Stefano, Cassani , Barbara, Fontana , Elena, Anselmo , Achille, Del Prete , Annalisa, Sozzani , Silvano, Vezzoni , Paolo, Poliani , Pietro L, Villa , Anna Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration. J Leukoc Biol, 2013-09-19;94(6):1221-30. 2013-09-19 [PMID: 24052573] (Mouse)
• Greenlee-Wacker MC, Briseno C, Galvan M, Moriel G, Velazquez P, Bohlson SS Membrane-associated CD93 regulates leukocyte migration and C1q-hemolytic activity during murine peritonitis. J. Immunol., 2011-08-17;187(6):3353-61. 2011-08-17 [PMID: 21849679] (Mouse)
• Jalili A, Pashenkov M, Kriehuber E, Wagner C, Nakano H, Stingl G, Wagner SN Induction of targeted cell migration by cutaneous administration of a DNA vector encoding a biologically active chemokine CCL21. J. Invest. Dermatol., 2010-02-25;130(6):1611-23. 2010-02-25 [PMID: 20182442] (Mouse)
• Demoor T, Bracke KR, Maes T, Vandooren B, Elewaut D, Pilette C, Joos GF, Brusselle GG Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis. Eur. Respir. J., 2009-01-22;34(2):405-16. 2009-01-22 [PMID: 19164352] (Mouse)
• Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses. J. Immunol., 2008-10-15;181(8):5360-7. 2008-10-15 [PMID: 18832692] (Mouse)
• Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice. Am. J. Pathol., 2007-04-01;170(4):1152-64. 2007-04-01 [PMID: 17392156] (Mouse)

FAQs for CCL21/6Ckine (DY457). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ccl21a