![Western Blot: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MORC2 antibody [NB600-236] used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MORC2-Antibody-Western-Blot-NB600-236-img0003.jpg "Western Blot: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MORC2 antibody [NB600-236] used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit MORC2 Antibody - BSA Free (NB600-236) is a polyclonal antibody validated for use in WB and IP. Anti-MORC2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | A synthetic peptide, which maps to the region between residues 600-700 of human zinc finger, CW-type with coiled-coil domain 1 using the numbering given in entry NP_055756.1 (GeneID 22880). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MORC2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | By Western Blot, this antibody recognizes a band at ~111 kDa, representing ZCWCC1 protein. May be used for immunoprecipitation, as well. Suggested working dilutions: * Western Blot - 1:2,500-1:10,000 Immunoprecipitation - 2-5 ug/mg lysate Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Western Blot GRB7 Antibody [Unconjugated]](https://images.novusbio.com/images2/GRB7_AF4856_Western_Blot_5571.jpg)
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| Publication using NB600-236 | Applications | Species |
|---|---|---|
| Shao Y, Li Y, Zhang J et al. Involvement of histone deacetylation in MORC2-mediated down-regulation of carbonic anhydrase IX. Nucleic Acids Res 2010-05-01 [PMID: 20110259] |
Secondary Antibodies |
Isotype Controls |
Research Areas for MORC2 Antibody (NB600-236)Find related products by research area.
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| Gene Symbol | MORC2 |
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| Uniprot |
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![Immunoprecipitation: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MORC2 antibody NB600-236 used for IP at 3 ug per reaction. MORC2 was also immunoprecipitated by rabbit anti-MORC2 antibody NB600-237. For blotting immunoprecipitated MORC2, NB600-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MORC2-Antibody-Immunoprecipitation-NB600-236-img0004.jpg "Immunoprecipitation: MORC2 Antibody [NB600-236] - Detection of human MORC2 (ZCWCC1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MORC2 antibody NB600-236 used for IP at 3 ug per reaction. MORC2 was also immunoprecipitated by rabbit anti-MORC2 antibody NB600-237. For blotting immunoprecipitated MORC2, NB600-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Western Blot Nestin Antibody (196908) [Unconjugated]](https://images.novusbio.com/images/mab1259_human-nestin-mab-clone-196908-41202410481129.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")