Monoglyceride Lipase Antibody

Western Blot: Monoglyceride Lipase Antibody [NBP2-19380] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Monoglyceride lipase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP2-19380] - Rat fore brain. Monoglyceride lipase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP2-19380] - U373 xenograft. Monoglyceride lipase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Monoglyceride Lipase Antibody [NBP2-19380] - Mouse esophagus. Monoglyceride lipase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Monoglyceride Lipase Antibody Summary

• Description: Novus Biologicals Rabbit Monoglyceride Lipase Antibody (NBP2-19380) is a polyclonal antibody validated for use in IHC and WB. Anti-Monoglyceride Lipase Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Monoglyceride Lipase. The exact sequence is proprietary.
• Predicted Species: Porcine (91%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MGLL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Monoglyceride Lipase Antibody

• HUK5
• HU-K5EC 3.1.1.23
• Lysophospholipase homolog
• Lysophospholipase-like
• MAGL
• MGLHUK5
• MGLL
• Monoacylglycerol lipase
• Monoglyceride Lipase

Background

Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Monoglyceride Lipase Antibody (NBP2-19380)(1)

Publication using NBP2-19380

• Kirkedal C, Elfving B, Muller HK et al. Hemisphere-dependent endocannabinoid system activity in prefrontal cortex and hippocampus of the Flinders Sensitive Line rodent model of depression Neurochem. Int. 2019-02-01 [PMID: 30716357] (WB, Rat)

Bioinformatics

• Gene Symbol: MGLL
• Uniprot:
  • Q99685()