MMR/CD206/Mannose Receptor Antibody (15-2)

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Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr
Clone
15-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

MMR/CD206/Mannose Receptor Antibody (15-2) Summary

Immunogen
Purified human mannose receptor from human placental tissue
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MRC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10-1:100
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
Application Notes
For IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum. For FC: Antibody 15-2 stains the extracellular domain of MR. WB: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa. Functional Studies: Antibody 15-2 functions as an inhibitor of binding. The antibody was functionally tested by preincubation of immobilized receptor with antibody. This prevented binding of t-PA to immobilized receptor.

Reactivity Notes

Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for MMR/CD206/Mannose Receptor Antibody (15-2)

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MRC1
Entrez
Uniprot