MMR/CD206/Mannose Receptor Antibody (15-2)

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - Frozen sections of human tonsil. NB600-1415 was used ina dilution of 1/25 and stains endothelia of lymph vesselsstrongly.

Immunohistochemistry-Frozen: MMR/CD206/Mannose Receptor Antibody (15-2) [NB600-1415] - staining Mannose Receptor in human mammary gland tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with 70% Ethanol and blocking for 10 minutes at RT was performed. The sample was incubated with primary antibody (1/10) for 1 hour. A HRP-conjugated mouse polyclonal to mouse IgG was used undiluted as secondary antibody.

• Reactivity: Hu
• Applications: WB, Flow, Func, IHC
• Clone: 15-2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

MMR/CD206/Mannose Receptor Antibody (15-2) Summary

• Additional Information: Clone 15-2 was used by HLDA to establish CD designation.
• Immunogen: Purified human mannose receptor from human placental tissue
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:100
  • Western Blot 1:10-1:100
• Application Notes: For IHC-F: Tissue sections were fixed in acetone/chloroform. Sections were preincubated with normal rabbit serum. For FC: Antibody 15-2 stains the extracellular domain of MR. WB: A non-reduced sample treatment and 6% SDS-Page was used. The band size is 175 kDa.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS containing 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for MMR/CD206/Mannose Receptor Antibody (15-2)

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail.The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody 15-2 prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody 15-2 can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (NB600-1415)(2)

Publications using NB600-1415

• Diep YN, Park HJ, Kwon JH et al. Astrocytic scar restricting glioblastoma via glutamate-MAO-B activity in glioblastoma-microglia assembloid Biomaterials research 2023-07-19 [PMID: 37468961] (IHC-P, Human)
• J Park, I Wetzel, I Marriott, D Dréau, C D'Avanzo, DY Kim, RE Tanzi, H Cho A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease Nat. Neurosci., 2018-06-27;21(7):941-951. 2018-06-27 [PMID: 29950669]

Bioinformatics

• Gene Symbol: MRC1
• Entrez:
  • 4360(Human)
• Uniprot:
  • P22897()