MMP-9 Antibody (CL0538) - BSA Free

Western Blot: MMP-9 Antibody (CL0538) [NBP2-52948] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MMP9 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human uterus shows absence of immunoreactivity.

Immunohistochemistry: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human duodenum shows strong cytoplasmic positivity in a subset of lymphoid cells.

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human lung shows strong positivity in lymphoid cells.

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human placenta shows strong immunoreactivity in granulocytes.

Immunohistochemistry-Paraffin: MMP-9 Antibody (CL0538) [NBP2-52948] - Staining of human tonsil shows strong immunoreactivity in a subset of lymphoid cells, primarily outside germinal center.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0538
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MMP-9 Antibody (CL0538) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from P14780, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  MMP-9 Recombinant Protein Antigen (NBP2-52948PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MMP-9 Antibody (CL0538) - BSA Free

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MMP-9 Antibody (NBP2-52948). (Showing 1 - 1 of 1 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9