MMP-3 Antibody (50647) [Unconjugated]

Western blot shows Recombinant Human MMP-3 Westrern Blot Standard Protein (2 µL, Catalog # WBC015). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-3 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-3 was detected in immersion fixed paraffin-embedded sections of normal human prostate and human prostate cancer tissue using Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells (right panel). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-3 was detected in immersion fixed MG-63 human osteosarcoma cell line using 10 µg/mL Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, IHC, CyTOF-ready, Flow, ICC/IF
• Clone: 50647
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MMP-3 Antibody (50647) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human MMP‑3
  Tyr18-Cys477
  Accession # P08254
• Specificity: Detects both the pro and active forms of human MMP-3 in direct ELISAs and Western blots. In direct ELISAs, 50‑100% cross-reactivity with recombinant mouse MMP-3 is observed, 10% cross-reactivity with recombinant human (rh) MMP-10 is observed and no cross-reactivity with rhMMP-1, -2, -7, -8, -9, -12 or -13 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-3 Antibody (50647) [Unconjugated]

• CHDS6
• EC 3.4.24
• EC 3.4.24.17
• matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• matrix metalloproteinase 3 (stromelysin 1, progelatinase)
• Matrix metalloproteinase-3
• MGC126102
• MGC126103
• MMP3
• MMP-3
• proteoglycanase
• SL-1
• STMY
• STMY1MGC126104
• STR1
• Stromelysin 1
• stromelysin-1
• transin-1

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha  chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta , IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-3 Antibody (MAB513)(4)

Publications using MAB513

• Jinsung Park, Hyosun Park, Young Lim Lee, Subin Weon, Yong-Gil Kim, Jae-Hyuk Yang et al. Blocking TNF alpha attenuates progressive cartilage matrix degradation in inflammatory arthritis Experimental and Therapeutic Medicine 5/27/2021 [PMID: 34093764]
• Villeneuve J, Block A, Le Bousse-Kerdiles MC, Lepreux S, Nurden P, Ripoche J, Nurden AT Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins. Exp. Hematol., 2009-05-03;37(7):849-56. 2009-05-03 [PMID: 19410025] (ICC, Human)
• Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. J. Immunol., 2007-05-01;178(9):5957-65. 2007-05-01 [PMID: 17442980] (Western Blot, Human)
• Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21. Gut, 2006-05-08;55(12):1774-80. 2006-05-08 [PMID: 16682426] (Western Blot, Human)

Review for MMP-3 Antibody (MAB513) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/11/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human
• Lot: IFN051705B

Bioinformatics

• Gene Symbol: MMP3
• Uniprot:
  • P08254()