Mouse MMP-2 ELISA Kit

Product Discontinued

Mouse MMP-2 ELISA Kit Summary

• Immunogen: The matrix metalloproteinases (MMPs) are a large family of zinc endopeptidases. All MMPs are synthesized as inactive proenzymes. The activation of these proenzymes is a critical step that leads to degradation of extracellular matrix components such as fibronectin and collagen type III. MMP2 is activated by other MMPs such as MMP14 and MMP16. MMP2 is known to degrade collagen types IV, V, VII and X as well as gelatin type I.
• Specificity: Mouse MMP-2
• Kit Type: ELISA Kit
• Gene: MMP2

Applications/Dilutions

• Dilutions:
  • ELISA 156pg/ml-10000pg/ml
• Application Notes: This product is useful for Sandwich ELISA detection. Strip format Biosensis ELISA kits offer the highest level of sensitivity available Strip 96-well format, use only what you need: High sensitivity and low blank Suitability for serum & tissues Reproducible results This kit contains: Pre-coated plates, Prepared standards Buffers, Detection reagents, Substrate Precise methods/instructions.

Reactivity Notes

Mouse

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This kit contains 2.0 x 96.0 Well Strip.

Alternate Names for Mouse MMP-2 ELISA Kit

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

The mouse MMP-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse MMP-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known MMP-2 concentration are added to these wells and allowed to complex with the bound MMP-2 antibody. A biotinylated mouse MMP-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of MMP-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse MMP-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MMP2