MMP-19 Antibody

Western Blot: MMP-19 Antibody [NBP2-17311] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP19 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MMP-19 Antibody [NBP2-17311] - Human hepatoma. MMP19 antibody dilution: 1:500. Scale bar = 100 um. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MMP-19 Antibody Summary

• Description: Novus Biologicals Rabbit MMP-19 Antibody (NBP2-17311) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MMP-19 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MMP-19. The exact sequence is proprietary.
• Localization: Secreted; extracellular space; extracellular matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MMP19
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Application Notes: Use in ELISA reported in scientific literature (PMID:31439546).
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31439546).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MMP-19 Antibody

• EC 3.4.24.-
• matrix metallopeptidase 19
• matrix metalloproteinase 18
• matrix metalloproteinase 19
• Matrix metalloproteinase RASI
• Matrix metalloproteinase-18
• matrix metalloproteinase-19
• MMP-18
• MMP18MMP-19
• MMP19
• MMP-19
• RASI
• RASI-1

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein is expressed in human epidermis and it has a role in cellular proliferation as well as migration and adhesion to type I collagen. Multiple transcript variants encoding distict isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MMP-19 Antibody (NBP2-17311)(1)

Publication using NBP2-17311

• Shang S, Ji X, Zhang L et al. Macrophage ABHD5 suppresses NF-kappa B-dependent matrix metalloproteinase expression and cancer metastasis Cancer Res. 2019-08-22 [PMID: 31439546] (KD, ELISA, Mouse)

Review for MMP-19 Antibody (NBP2-17311) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Shinford Sun WB Human 09/16/2024

Summary

• Application: Western Blot
• Sample Tested: human lung cancer cell line
• Species: Human
• Lot: 40744

Bioinformatics

• Gene Symbol: MMP19