Recombinant Human MMP-1 His Protein

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SDS-Page: Recombinant Human MMP-1 Protein [NBP2-52617]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human MMP-1 His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-469 of Human MMP-1

Source: Baculovirus

Amino Acid Sequence: HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNP RWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHF DEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAI TTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KNLEHHHHHH

Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Gene
MMP1
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
53.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM MES buffer (pH 5.5), 10 mM CaCl2, 100 mM NaCl, 0.05% Brij35, 30% glycerol
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human MMP-1 His Protein

  • CLGmatrix metalloprotease 1
  • CLGN
  • EC 3.4.24
  • EC 3.4.24.7
  • Fibroblast collagenase
  • interstitial collagenase
  • matrix metallopeptidase 1 (interstitial collagenase)
  • matrix metalloproteinase 1 (interstitial collagenase)
  • Matrix metalloproteinase-1
  • MMP1
  • MMP-1

Background

MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Recombinant human MMP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol MMP1
Uniprot