MMP-1 Antibody (3B6)

Product Discontinued

MMP-1 Antibody (3B6) Summary

• Immunogen: The antibody reacts with the MMP-1 antigen.
• Specificity: This monoclonal antibody binds to human MMP-1. Reactivity in other species has not been determined. The antibody was tested for cross-reactivity with MMP-2, MMP-3, and MMP-9 and did not cross-react. The 3B6 monoclonal antibody was generated from mice immunized with a 12 mer-peptide derived from the sequence of human MMP-1. The amino acid sequence of this peptide was: CSSFGFPRTVKH (Murray et al., 1998a and 1998b).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
• Application Notes: The 3B6 monoclonal antibody has been tested for use in Western blotting and immunohistochemical analysis of formalin-fixed/paraffin-embedded tissues. It is suggested to use colon carcinoma as positive control. Antigen retrieval by microwave treatment (5-20 min at full power, 950 W in 0.01 M sodium citrate buffer, pH 6.0) is recommended for immunohistochemical analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Hybridoma culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for MMP-1 Antibody (3B6)

• CLGmatrix metalloprotease 1
• CLGN
• EC 3.4.24
• EC 3.4.24.7
• Fibroblast collagenase
• interstitial collagenase
• matrix metallopeptidase 1 (interstitial collagenase)
• matrix metalloproteinase 1 (interstitial collagenase)
• Matrix metalloproteinase-1
• MMP1
• MMP-1

Background

Human interstitial collagenase (matrix metalloproteinase-1, MMP-1), an enzyme whose only known physiologic substrate has heretofore been believed to be the extracellular matrix molecule, collagen. Data indicate that matrix metalloproteinase-1 displays an expanded substrate repertoire that supports the existence of a new interface between connective tissue turnover and serine proteinase inhibitors (1). It has been shown that the MMP-1 functions as a protease agonist of Protease-activated receptors (PAR1) cleaving the receptor at the proper site to generate PAR1-dependent Ca2+ signals and migration. These results demonstrate that MMP-1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion (2). It has also been suggested that increased levels of MMP-1 due to tobacco smoking plays a major role in the aging process of skin since MMP-1 degrades collagen, which accounts for at least 70% of the dry weight of dermis. Significantly more MMP-1 has been detected in the skin of smokers than non-smokers whereas no difference was seen for the tissue inhibitor of metalloproteinases 1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MMP1