MMP-1 Antibody (3B6)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clone
3B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MMP-1 Antibody (3B6) Summary

Immunogen
The antibody reacts with the MMP-1 antigen.
Specificity
This monoclonal antibody binds to human MMP-1. Reactivity in other species has not been determined. The antibody was tested for cross-reactivity with MMP-2, MMP-3, and MMP-9 and did not cross-react. The 3B6 monoclonal antibody was generated from mice immunized with a 12 mer-peptide derived from the sequence of human MMP-1. The amino acid sequence of this peptide was: CSSFGFPRTVKH (Murray et al., 1998a and 1998b).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MMP1
Purity
Tissue culture supernatant
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry-Paraffin
Application Notes
The 3B6 monoclonal antibody has been tested for use in Western blotting and immunohistochemical analysis of formalin-fixed/paraffin-embedded tissues. It is suggested to use colon carcinoma as positive control. Antigen retrieval by microwave treatment (5-20 min at full power, 950 W in 0.01 M sodium citrate buffer, pH 6.0) is recommended for immunohistochemical analysis.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Hybridoma culture supernatant
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MMP-1 Antibody (3B6)

  • CLGmatrix metalloprotease 1
  • CLGN
  • EC 3.4.24
  • EC 3.4.24.7
  • Fibroblast collagenase
  • interstitial collagenase
  • matrix metallopeptidase 1 (interstitial collagenase)
  • matrix metalloproteinase 1 (interstitial collagenase)
  • Matrix metalloproteinase-1
  • MMP1
  • MMP-1

Background

Human interstitial collagenase (matrix metalloproteinase-1, MMP-1), an enzyme whose only known physiologic substrate has heretofore been believed to be the extracellular matrix molecule, collagen. Data indicate that matrix metalloproteinase-1 displays an expanded substrate repertoire that supports the existence of a new interface between connective tissue turnover and serine proteinase inhibitors (1). It has been shown that the MMP-1 functions as a protease agonist of Protease-activated receptors (PAR1) cleaving the receptor at the proper site to generate PAR1-dependent Ca2+ signals and migration. These results demonstrate that MMP-1 in the stromal-tumor microenvironment can alter the behavior of cancer cells through PAR1 to promote cell migration and invasion (2). It has also been suggested that increased levels of MMP-1 due to tobacco smoking plays a major role in the aging process of skin since MMP-1 degrades collagen, which accounts for at least 70% of the dry weight of dermis. Significantly more MMP-1 has been detected in the skin of smokers than non-smokers whereas no difference was seen for the tissue inhibitor of metalloproteinases 1 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MMP1
Entrez