MKLP1 Antibody

Western Blot: MKLP1 Antibody [NBP2-17299] - Sample (30 ug of whole cell lysate) A: HeLa 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MKLP1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MKLP1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (91%), Rat (90%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF23
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MKLP1 Antibody

• kinesin family member 23
• kinesin-like 5 (mitotic kinesin-like protein 1)
• Kinesin-like protein 5
• kinesin-like protein KIF23
• KNSL5CHO1
• mitotic kinesin-like 1
• MKLP-1
• MKLP1Mitotic kinesin-like protein 1

Background

The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in two transcript variants encoding two different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KIF23
• Uniprot:
  • Q02241(Human)