KIF20A Antibody - BSA Free

Western Blot: KIF20A Antibody [NBP1-87176] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.

Western Blot: KIF20A Antibody [NBP1-87176] - Analysis in control (vector only transfected HEK293T lysate) and KIF20A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: KIF20A Antibody [NBP1-87176] - Staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

KIF20A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit KIF20A Antibody - BSA Free (NBP1-87176) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFAL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF20A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  KIF20A Protein (NBP1-87176PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for KIF20A Antibody - BSA Free

• FLJ21151
• GG10_2
• kinesin family member 20A
• kinesin-like protein KIF20A
• mitotic kinesin-like protein 2
• MKLP2
• Rab6-interacting kinesin-like protein
• RAB6KIFLkinesin-like (rabkinesin6)
• rabkinesin-6

Background

KIF20A is a member of the kinesin-like protein family and has 1 kinesin motor domain. It interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B and may act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. KIF20A has a microtubule plus end-directed motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF20A
• Entrez:
  • 10112(Human)
• Uniprot:
  • O95235(Human)